Abstract：
UNASSIGNED: Although the diverse communities of tick-borne viruses (TBVs) have recently been proposed, the threat of infection and exposure to TBVs among humans across Kenya has been poorly understood.
UNASSIGNED: Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne viral agent associated with the epidemic of severe fever with thrombocytopenia syndrome (SFTS) disease in East Asian countries. This study investigated the seroprevalence of SFTSV among humans in Kenya.
UNASSIGNED: Serum samples were collected from 459 healthy people in Kenya and tested for anti-SFTSV antibodies, which were further confirmed by immunofluorescence assays. Micro neutralization assays were performed to identify neutralising antibodies against SFTSV and SFTSV-related viruses.
UNASSIGNED: A high seroprevalence (162/459, 35.3%) of SFTSV was found in the samples from nine of the ten surveyed counties in Kenya, with higher rates in the eastern plateau forelands, semiarid and arid areas, and coastal areas than in the area aside Rift valley. The seropositive rate was slightly higher in women than in men and was significantly higher in the 55-64 age group. Neutralising activity against SFTSV was detected in four samples, resulting in a rate of 0.9%. No cross-neutralising activity against the SFTSV-related Guertu virus and Heartland virus was detected in the anti-SFTSV positive serum samples.
UNASSIGNED: The results provide serologic evidence of human exposure to SFTSV in Kenya and extend our understanding of SFTSV prevalence from Asia to Africa. The findings suggest an increasing threat of exposure to emerging TBVs and the need to investigate tick viromes in Kenya.
UNASSIGNED: Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne viral agent associated with the epidemic of severe fever with thrombocytopenia syndrome (SFTS) disease in East Asian countries. This study investigated the seroprevalence of SFTSV among humans in Kenya.
UNASSIGNED: Serum samples were collected from 459 healthy people in Kenya and tested for anti-SFTSV antibodies, which were further confirmed by immunofluorescence assays. Micro neutralization assays were performed to identify neutralising antibodies against SFTSV and SFTSV-related viruses.
UNASSIGNED: A high seroprevalence (162/459, 35.3%) of SFTSV was found in the samples from nine of the ten surveyed counties in Kenya, with higher rates in the eastern plateau forelands, semiarid and arid areas, and coastal areas than in the area aside Rift valley. The seropositive rate was slightly higher in women than in men and was significantly higher in the 55-64 age group. Neutralising activity against SFTSV was detected in four samples, resulting in a rate of 0.9%. No cross-neutralising activity against the SFTSV-related Guertu virus and Heartland virus was detected in the anti-SFTSV positive serum samples.
UNASSIGNED: The results provide serologic evidence of human exposure to SFTSV in Kenya and extend our understanding of SFTSV prevalence from Asia to Africa. The findings suggest an increasing threat of exposure to emerging TBVs and the need to investigate tick viromes in Kenya.
摘要：
■尽管最近提出了蜱传病毒（TBV）的多样性群落，对肯尼亚各地人类感染和接触TBV的威胁知之甚少.
■严重发热伴血小板减少综合征病毒（SFTSV）是东亚国家与严重发热伴血小板减少综合征（SFTS）疾病的流行有关的新兴蜱传病毒。这项研究调查了肯尼亚人群中SFTSV的血清阳性率。
■从肯尼亚的459名健康人中收集血清样本，并测试抗SFTSV抗体，免疫荧光分析进一步证实了这一点。进行微中和测定以鉴定针对SFTSV和SFTSV相关病毒的中和抗体。
■在肯尼亚10个调查县的9个样本中发现了高血清阳性率（162/459，35.3%）的SFTSV，东部高原前陆的发病率较高，半干旱和干旱地区，和沿海地区比裂谷以外的地区。女性血清阳性率略高于男性，在55-64岁年龄段明显高于男性。在四个样品中检测到针对SFTSV的中和活性，产生0.9%的比率。在抗SFTSV阳性血清样品中未检测到针对SFTSV相关的Guertu病毒和Heartland病毒的交叉中和活性。
■结果提供了肯尼亚人暴露于SFTSV的血清学证据，并将我们对SFTSV患病率的理解从亚洲扩展到非洲。研究结果表明，暴露于新兴TBV的威胁越来越大，并且需要在肯尼亚调查tick病毒。
■严重发热伴血小板减少综合征病毒（SFTSV）是东亚国家与严重发热伴血小板减少综合征（SFTS）疾病的流行有关的新兴蜱传病毒。这项研究调查了肯尼亚人群中SFTSV的血清阳性率。
■从肯尼亚的459名健康人中收集血清样本，并测试抗SFTSV抗体，免疫荧光分析进一步证实了这一点。进行微中和测定以鉴定针对SFTSV和SFTSV相关病毒的中和抗体。
■在肯尼亚10个调查县的9个样本中发现了高血清阳性率（162/459，35.3%）的SFTSV，东部高原前陆的发病率较高，半干旱和干旱地区，和沿海地区比裂谷以外的地区。女性血清阳性率略高于男性，在55-64岁年龄段明显高于男性。在四个样品中检测到针对SFTSV的中和活性，产生0.9%的比率。在抗SFTSV阳性血清样品中未检测到针对SFTSV相关的Guertu病毒和Heartland病毒的交叉中和活性。
■结果提供了肯尼亚人暴露于SFTSV的血清学证据，并将我们对SFTSV患病率的理解从亚洲扩展到非洲。研究结果表明，暴露于新兴TBV的威胁越来越大，并且需要在肯尼亚调查tick病毒。
