PDF(Pubmed)
Abstract：
Here, we report the generation of a transgenic Lifeact-EGFP quail line for the investigation of actin organization and dynamics during morphogenesis in vivo. This transgenic avian line allows for the high-resolution visualization of actin structures within the living embryo, from the subcellular filaments that guide cell shape to the supracellular assemblies that coordinate movements across tissues. The unique suitability of avian embryos to live imaging facilitates the investigation of previously intractable processes during embryogenesis. Using high-resolution live imaging approaches, we present the dynamic behaviors and morphologies of cellular protrusions in different tissue contexts. Furthermore, through the integration of live imaging with computational segmentation, we visualize cells undergoing apical constriction and large-scale actin structures such as multicellular rosettes within the neuroepithelium. These findings not only enhance our understanding of tissue morphogenesis but also demonstrate the utility of the Lifeact-EGFP transgenic quail as a new model system for live in vivo investigations of the actin cytoskeleton.
摘要：
这里,我们报告了转基因Lifeact-EGFP鹌鹑系的产生，用于研究体内形态发生过程中的肌动蛋白组织和动力学。这种转基因禽系允许活胚胎内肌动蛋白结构的高分辨率可视化，从引导细胞形状的亚细胞细丝到协调跨组织运动的上细胞组件。禽类胚胎对活成像的独特适用性有助于研究胚胎发生过程中先前难以处理的过程。使用高分辨率实时成像方法，我们介绍了细胞突起在不同组织环境中的动态行为和形态。此外，通过将实时成像与计算分割相结合，我们可以观察到细胞经历顶端收缩和大规模的肌动蛋白结构，如神经上皮内的多细胞玫瑰花结。这些发现不仅增强了我们对组织形态发生的理解，而且证明了Lifeact-EGFP转基因鹌鹑作为肌动蛋白细胞骨架活体体内研究的新模型系统的实用性。
