Abstract：
To examine the relationship between gut microbiota and disease development in chronic heart failure patients with different nutritional risk. The study analyzed stool samples from 62 CHF patients and 21 healthy peoples using 16S rRNA gene sequencing. CHF patients were separated into risk (n = 30) and non-risk group (n = 32) based on NRS2002 scores. Analysis methods used were LEfSe, random forest regression model, ROC curves, BugBase, PICRUSt2, metagenomeSeq. Risk group includes 11 cases of HFrEF, 6 cases of HFpEF, and 13 cases of HFmrEF. LefSe analysis confirmed that the risk group had higher levels of Enterobacter and Escherichia-Shigella. Correlation analysis revealed a negative correlation between prealbumin and Escherichia-Shigella. The presence of Enterobacter and Escherichia-Shigella worsens intestinal inflammation in CHF patients, impacting lysine metabolism by influencing its degradation metabolic function. This interference further disrupts albumin and prealbumin synthesis, leading to malnutrition in CHF patients and ultimately worsening the disease.
摘要：
探讨不同营养风险的慢性心力衰竭患者肠道菌群与疾病发展的关系。该研究使用16SrRNA基因测序分析了来自62名CHF患者和21名健康人群的粪便样本。根据NRS2002评分将CHF患者分为风险组（n=30）和非风险组（n=32）。使用的分析方法是LEfSe，随机森林回归模型，ROC曲线,BugBase,PICRUSt2，宏基因组序列。风险组包括11例HFrEF，6例HFpEF，HFmrEF13例。LefSe分析证实，风险组的肠杆菌和大肠杆菌志贺氏菌水平较高。相关分析显示前白蛋白与大肠杆菌志贺氏菌呈负相关。肠杆菌和大肠杆菌-志贺氏菌的存在恶化CHF患者的肠道炎症，通过影响赖氨酸的降解代谢功能来影响赖氨酸的代谢。这种干扰进一步破坏白蛋白和前白蛋白的合成，导致CHF患者营养不良，并最终使疾病恶化。
