Abstract：
Pancreatic islet transplantation is an emerging treatment for type I diabetes; however, it is limited by donor matching and availability. Porcine islet xenotransplantation offers a promising alternative to allotransplantation, with the potential for large-scale production of on-demand, functional islets. The yield and viability of isolated islets is highly susceptible to the quality of the donor pancreas and the method of procurement, particularly the duration of warm-ischemia time. To improve organ preservation and subsequent islet yield and viability, we have developed a protocol for surgical perfusion and resection of the porcine pancreas. This protocol employs direct infrarenal aortic cannulation and organ perfusion to both minimize warm-ischemia time and simplify the procedure for operators who do not have extensive surgical expertise. Subsequent arterial perfusion of the pancreas via the aorta flushes stagnant blood from the microvasculature, thereby reducing thrombosis and oxidative damage to the tissue. This manuscript provides a detailed protocol for surgical perfusion and resection of the porcine pancreas, followed by islet isolation and purification.
摘要：
胰岛移植是I型糖尿病的新兴治疗方法；然而，它受到捐助者匹配和可用性的限制。猪胰岛异种移植为同种异体移植提供了一种有希望的替代方法，随着按需大规模生产的潜力，功能性胰岛。分离的胰岛的产量和生存能力极易受到供体胰腺质量和采购方法的影响,特别是热缺血时间的持续时间。为了提高器官保存和随后的胰岛产量和生存能力，我们制定了猪胰腺的手术灌注和切除方案.该方案采用直接肾下主动脉插管和器官灌注，以最大程度地减少热缺血时间，并简化没有广泛外科专业知识的操作员的手术程序。随后通过主动脉对胰腺的动脉灌注冲刷了微脉管系统中的停滞血液，从而减少对组织的血栓形成和氧化损伤。该手稿提供了猪胰腺手术灌注和切除的详细方案，随后进行胰岛分离和纯化。
