PDF(Pubmed)
Abstract：
Studies have shown that a lot of traditional Chinese medicines could improve the immunity of the body. Dangdi oral liquid (DDO) was mainly composed of Angelica sinensis (Oliv.) Diels (Danggui), Rehmannia glutinosa Libosch. (Dihuang), Achyranthes bidentata Bl. (Niuxi), Glycyrrhiza uralensis Fisch. (Gancao). In this study, the rapid ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS) method was used to identify the potentially effective compounds of DDO. Then the immune activity of DDO was measured by lymphocyte proliferation, macrophage phagocytic function, NK cell activity, delayed type hypersensitivity reaction, hemolytic plaque number, sIgA content and immune organ index. The results showed that a total of 51 compounds were identified. In addition, DDO could significantly promote the lymphocyte proliferation, improve macrophage phagocytic ability, NK cell activity, hemolytic plaque number, sIgA content and immune organ index compared with control group, and the medium dose possessed the best efficacy (P＜0.05). These results indicated that DDO could enhance the immunity of mice.
摘要：
研究表明，许多中药可以提高人体的免疫力。当迪口服液（DDO）主要由当归（Oliv。)迪尔斯(当归)，地黄。（地黄），牛膝Bl.（牛溪），甘草。（甘草）。在这项研究中,采用快速超高效液相色谱-四极杆飞行时间质谱(UPLC-Q-TOF/MS)法鉴定DDO潜在有效化合物。然后通过淋巴细胞增殖测定DDO的免疫活性，巨噬细胞吞噬功能，NK细胞活性，迟发型超敏反应,溶血菌斑数,sIgA含量和免疫器官指数。结果表明，共鉴定出51个化合物。此外,DDO能显著促进淋巴细胞增殖,提高巨噬细胞吞噬能力，NK细胞活性，溶血菌斑数,sIgA含量及免疫器官指数与对照组比较,中剂量组疗效最佳(P＜0.05)。这些结果表明DDO可以增强小鼠的免疫力。
