PDF(Pubmed)
Abstract：
UNASSIGNED: Toxoplasma gondii, a neurotropic protozoan, infects up one to third of the world population. The parasite can invade a wide variety of nucleated cells but preferably glial cells. Glia maturation factor β (GMFβ), a 17KD protein expressed at high levels in the central nervous system is predominantly related to neurodegenerative diseases such as Alzheimer\'s disease, Parkinson\'s disease, and Multiple sclerosis. We aimed to determine the expression level of GMFβ and its relation to other pro-inflammatory factors (IL33, SDF1, and CCL2) on T. gondii infected human neuroblastoma cell line.
UNASSIGNED: The human neuroblastoma (SK_NMC C535) cell line was infected by 5×106 (1:1 ratio). The supernatant was collected after cell lysis and centrifugation. Total RNA was extracted using the Yekta Tajhiz RNA extraction kit. cDNA was synthesized based on RevertAid First Strand cDNA Synthesis Kit manufacturer\'s protocol (Parstous, cDNA synthesis kit, Iran). The specificity of each primer pair (GMFβ, IL33, SDF1, and CCL2) was provided by NCBI BLAST. Gene expression level was measured using Real-Time PCR. All experiments were conducted at the Hamadan University of Medical Sciences, western Iran in 2022.
UNASSIGNED: The GMFβ increased significantly up to 1.35-fold (P=0.007). The increase in GMFβ expression in neuroblastoma cells was consistent with the increase in pro-inflammatory factors (CCL2 (0.47), IL33 (0.152) and, SDF1 (1.33)).
UNASSIGNED: GMFβ upregulation can be a novel indicator of the destruction of nerve cells.
UNASSIGNED: The human neuroblastoma (SK_NMC C535) cell line was infected by 5×106 (1:1 ratio). The supernatant was collected after cell lysis and centrifugation. Total RNA was extracted using the Yekta Tajhiz RNA extraction kit. cDNA was synthesized based on RevertAid First Strand cDNA Synthesis Kit manufacturer\'s protocol (Parstous, cDNA synthesis kit, Iran). The specificity of each primer pair (GMFβ, IL33, SDF1, and CCL2) was provided by NCBI BLAST. Gene expression level was measured using Real-Time PCR. All experiments were conducted at the Hamadan University of Medical Sciences, western Iran in 2022.
UNASSIGNED: The GMFβ increased significantly up to 1.35-fold (P=0.007). The increase in GMFβ expression in neuroblastoma cells was consistent with the increase in pro-inflammatory factors (CCL2 (0.47), IL33 (0.152) and, SDF1 (1.33)).
UNASSIGNED: GMFβ upregulation can be a novel indicator of the destruction of nerve cells.
摘要：
■弓形虫，亲神经的原生动物,感染了世界人口的三分之一。寄生虫可侵入多种有核细胞,但优选神经胶质细胞。胶质细胞成熟因子β(GMFβ),在中枢神经系统中高水平表达的17KD蛋白主要与神经退行性疾病如阿尔茨海默病有关，帕金森病,和多发性硬化症。我们旨在确定弓形虫感染的人神经母细胞瘤细胞系中GMFβ的表达水平及其与其他促炎因子（IL33，SDF1和CCL2）的关系。
■用5×106(1∶1比例)感染人神经母细胞瘤(SK_NMCC535)细胞系。细胞裂解和离心后收集上清液。使用YektaTajhizRNA提取试剂盒提取总RNA。cDNA是根据RevertAid第一链cDNA合成试剂盒制造商的方案合成的(Parstous，cDNA合成试剂盒，伊朗)。每个引物对的特异性(GMFβ，IL33、SDF1和CCL2)由NCBIBLAST提供。使用实时PCR测量基因表达水平。所有实验均在哈马丹医科大学进行，2022年伊朗西部。
■GMFβ显著增加至1.35倍(P=0.007)。神经母细胞瘤细胞中GMFβ表达的增加与促炎因子的增加一致(CCL2(0.47),IL33（0.152）和，SDF1(1.33))。
■GMFβ上调可以成为神经细胞破坏的一种新指标。
■用5×106(1∶1比例)感染人神经母细胞瘤(SK_NMCC535)细胞系。细胞裂解和离心后收集上清液。使用YektaTajhizRNA提取试剂盒提取总RNA。cDNA是根据RevertAid第一链cDNA合成试剂盒制造商的方案合成的(Parstous，cDNA合成试剂盒，伊朗)。每个引物对的特异性(GMFβ，IL33、SDF1和CCL2)由NCBIBLAST提供。使用实时PCR测量基因表达水平。所有实验均在哈马丹医科大学进行，2022年伊朗西部。
■GMFβ显著增加至1.35倍(P=0.007)。神经母细胞瘤细胞中GMFβ表达的增加与促炎因子的增加一致(CCL2(0.47),IL33（0.152）和，SDF1(1.33))。
■GMFβ上调可以成为神经细胞破坏的一种新指标。
