UNASSIGNED: We genotyped all known individuals using a 2b-RADseq approach, compared genetic diversity in wild populations and ex situ collections, and conducted parentage analysis of the collections.
UNASSIGNED: Wild populations were found to have greater levels of genetic diversity than ex situ populations as measured by number of private alleles, number of polymorphic sites, observed and expected heterozygosity, nucleotide diversity, and allelic richness. In addition, only 32.6% of wild individuals are represented ex situ and many individuals were found to be the product of selfing by a single wild individual.
UNASSIGNED: Population genomic analyses provided important insights into the conservation of genetic diversity in K. gigas, identifying gaps and inefficiencies, but also highlighting strategies to conserve genetic diversity ex situ. Genomic analyses provide essential information to ensure that collections effectively conserve genetic diversity in threatened tropical trees.
■我们使用2b-RADseq方法对所有已知个体进行了基因分型,比较了野生种群和异地采集的遗传多样性,并对藏品进行了亲子关系分析。
■根据私有等位基因的数量,发现野生种群比离地种群具有更高的遗传多样性水平,多态位点的数量,观察到的和预期的杂合性,核苷酸多样性,和等位基因丰富度。此外,只有32.6%的野生个体在异地代表,许多个体被发现是单个野生个体自交的产物。
■群体基因组分析为K.gigas遗传多样性的保护提供了重要的见解,找出差距和低效率,但也强调了保护遗传多样性的策略。基因组分析提供了必要的信息,以确保馆藏有效地保护受威胁的热带树木的遗传多样性。