PDF(Pubmed)
Abstract：
The domain of unknown function 668 (DUF668) is a gene family that may play a key role in plant growth and development as well as in responding to adversity coercion stresses. However, the DUF668 gene family has not yet been well identified and characterized in tomato. In this study, a total of nine putative SlDUF668 genes were identified in tomato, distributed on six chromosomes. Phylogenetic analyses revealed that SlDUF668 proteins were classified into two major groups. Members within the same group largely displayed analogous gene structure and conserved motif compositions. Several cis-elements were exhibited in the upstream sequences of the SlDUF668 genes, including elements implicated in plant growth and development processes, abiotic stress and hormone responses. Further, the study assessed the expression patterns of the SlDUF668 gene family in various tomato tissues, five plant hormones treatments, three abiotic stresses using qRT-PCR. The SlDUF668 genes expressed ubiquitously in various tissues, and five genes (SlDUF668-04, SlDUF668-06, SlDUF668-07, SlDUF668-08 and SlDUF668-09) showed tissue specificity. And SlDUF668 genes responded to abiotic stresses such as salt, drought and cold to varying degrees. Overall, our study provided a base for the tomato DUF668 gene family and laid a foundation for further understanding the functional characteristics of DUF668 genes in tomato plants.
摘要：
未知功能域668（DUF668）是一个基因家族，可能在植物的生长发育以及对逆境胁迫的响应中起关键作用。然而,DUF668基因家族尚未在番茄中得到很好的鉴定和表征。在这项研究中,在番茄中总共鉴定出9个推定的SlDUF668基因，分布在6条染色体上。系统发育分析显示SlDUF668蛋白被分为两大类。同一组中的成员在很大程度上表现出类似的基因结构和保守的基序组成。在SlDUF668基因的上游序列中显示了几个顺式元件，包括与植物生长和发育过程有关的元素，非生物胁迫和激素反应。Further,该研究评估了SlDUF668基因家族在各种番茄组织中的表达模式，五种植物激素治疗，使用qRT-PCR的三种非生物胁迫。SlDUF668基因在各种组织中普遍表达，5个基因(SlDUF668-04、SlDUF668-06、SlDUF668-07、SlDUF668-08和SlDUF668-09)显示组织特异性。SlDUF668基因对盐等非生物胁迫有反应，不同程度的干旱和寒冷。总的来说,本研究为番茄DUF668基因家族的研究奠定了基础,为进一步了解DUF668基因在番茄植株中的功能特性奠定了基础。
