UNASSIGNED: We first conducted a phylogenetic analysis to determine how the sequence of CpMRJP1 compares with other species. We then investigated how supplementing the diets of both diapausing and nondiapausing females of Cx. pipiens with royal jelly affects egg follicle length, fat content, protein content, starvation resistance, and metabolic profile.
UNASSIGNED: We found that feeding royal jelly to females reared in long-day, diapause-averting conditions significantly reduced the egg follicle lengths and switched their metabolic profiles to be similar to diapausing females. In contrast, feeding royal jelly to females reared in short-day, diapause-inducing conditions significantly reduced lifespan and switched their metabolic profile to be similar nondiapausing mosquitoes. Moreover, RNAi directed against CpMRJPI significantly increased egg follicle length of short-day reared females, suggesting that these females averted diapause.
UNASSIGNED: Taken together, our data show that consuming royal jelly reverses several key seasonal phenotypes of Cx. pipiens and that these responses are likely mediated in part by CpMRJP1.
■我们首先进行了系统发育分析,以确定CpMRJP1的序列与其他物种的比较。然后,我们研究了如何补充Cx的滞育和非滞育女性的饮食。pipiens与蜂王浆影响卵泡长度,脂肪含量,蛋白质含量,耐饥饿,和代谢概况。
■我们发现给雌性喂食蜂王浆在漫长的一天中饲养,防止滞育的条件显着减少了卵泡的长度,并将其代谢谱转换为与滞育雌性相似。相比之下,给短期饲养的雌性喂食蜂王浆,导致滞育的条件显着降低了寿命,并将其代谢特征转变为类似的非滞育蚊子。此外,针对CpMRJPI的RNAi显着增加了短日饲养雌性的卵泡长度,表明这些雌性避免了滞育。
■放在一起,我们的数据显示,食用蜂王浆逆转了Cx的几种关键季节性表型。Pipiens,这些反应可能部分由CpMRJP1介导。