PDF(Pubmed)
Abstract：
UNASSIGNED: The present study aimed to investigate plastic tubes without additives as alternatives to glass and silica-coated plastic tubes, in the production of PRF membranes.
UNASSIGNED: Nine blood samples were collected from eight volunteers (n = 8) separated into three groups, according to tube material: glass, silica-coated plastic, and plastic without additives. In each group, the samples were centrifuged using different relative centrifugation forces: L-PRF (700 g/12 min), A-PRF (200 g/14 min), and A-PRF + (200 g/8 min). The generated membranes were evaluated by histomorphometry, considering the fibrin network, platelet aggregates, and cellular morphology, by light microscopy. The ultrastructural cellular morphology integrity was evaluated by transmission electron microscopy.
UNASSIGNED: The L-PRF (p < 0.019) and A-PRF (p < 0.001) membranes showed a significantly lower fibrin network density in plastic tubes without additives compared to glass and silica-coated plastic tubes. Plastic tubes without additives revealed a significantly higher platelet percentage, regardless of the protocol (p < 0.005). In all groups, TEM analysis showed preserved normal morphological ultrastructure, maintaining the integrity of cellular components.
UNASSIGNED: Plastic tubes without additives offer a viable alternative for producing PRF membranes. They exhibited a higher platelet density and demonstrated fibrin network and cellular morphology similar to those of glass and silica-coated plastic tubes, irrespective of the centrifugation protocol.
UNASSIGNED: Nine blood samples were collected from eight volunteers (n = 8) separated into three groups, according to tube material: glass, silica-coated plastic, and plastic without additives. In each group, the samples were centrifuged using different relative centrifugation forces: L-PRF (700 g/12 min), A-PRF (200 g/14 min), and A-PRF + (200 g/8 min). The generated membranes were evaluated by histomorphometry, considering the fibrin network, platelet aggregates, and cellular morphology, by light microscopy. The ultrastructural cellular morphology integrity was evaluated by transmission electron microscopy.
UNASSIGNED: The L-PRF (p < 0.019) and A-PRF (p < 0.001) membranes showed a significantly lower fibrin network density in plastic tubes without additives compared to glass and silica-coated plastic tubes. Plastic tubes without additives revealed a significantly higher platelet percentage, regardless of the protocol (p < 0.005). In all groups, TEM analysis showed preserved normal morphological ultrastructure, maintaining the integrity of cellular components.
UNASSIGNED: Plastic tubes without additives offer a viable alternative for producing PRF membranes. They exhibited a higher platelet density and demonstrated fibrin network and cellular morphology similar to those of glass and silica-coated plastic tubes, irrespective of the centrifugation protocol.
摘要：
■本研究旨在研究不含添加剂的塑料管作为玻璃和二氧化硅涂层塑料管的替代品，在PRF膜的生产中。
■从8名志愿者（n=8）中收集9份血样，分为3组，根据管材料：玻璃，二氧化硅涂层塑料，和不含添加剂的塑料。在每一组中,使用不同的相对离心力离心样品：L-PRF（700g/12分钟），A-PRF（200g/14分钟），和A-PRF+(200g/8min)。通过组织形态计量学评估生成的膜，考虑到纤维蛋白网络，血小板聚集体,和细胞形态，通过光学显微镜。通过透射电子显微镜评估超微结构细胞形态的完整性。
与玻璃和二氧化硅涂覆的塑料管相比,L-PRF(p<0.019)和A-PRF(p<0.001)膜在没有添加剂的塑料管中显示出显著更低的纤维蛋白网络密度。没有添加剂的塑料管显示出明显更高的血小板百分比，无论方案如何（p<0.005）。在所有组中,TEM分析显示保存正常形态超微结构，保持细胞成分的完整性。
■不含添加剂的塑料管为生产PRF膜提供了可行的替代方案。它们表现出更高的血小板密度，并表现出与玻璃和二氧化硅涂层塑料管相似的纤维蛋白网络和细胞形态，无论离心协议。
■从8名志愿者（n=8）中收集9份血样，分为3组，根据管材料：玻璃，二氧化硅涂层塑料，和不含添加剂的塑料。在每一组中,使用不同的相对离心力离心样品：L-PRF（700g/12分钟），A-PRF（200g/14分钟），和A-PRF+(200g/8min)。通过组织形态计量学评估生成的膜，考虑到纤维蛋白网络，血小板聚集体,和细胞形态，通过光学显微镜。通过透射电子显微镜评估超微结构细胞形态的完整性。
与玻璃和二氧化硅涂覆的塑料管相比,L-PRF(p<0.019)和A-PRF(p<0.001)膜在没有添加剂的塑料管中显示出显著更低的纤维蛋白网络密度。没有添加剂的塑料管显示出明显更高的血小板百分比，无论方案如何（p<0.005）。在所有组中,TEM分析显示保存正常形态超微结构，保持细胞成分的完整性。
■不含添加剂的塑料管为生产PRF膜提供了可行的替代方案。它们表现出更高的血小板密度，并表现出与玻璃和二氧化硅涂层塑料管相似的纤维蛋白网络和细胞形态，无论离心协议。
