PDF(Pubmed)
Abstract：
Background: To better assess the peripheral immune status and aid in the early diagnosis and prognosis of tumors, we compared the proportion and absolute counting of peripheral immune cell subsets in healthy individuals and tumor patients of varying ages, taking into account the impact of sex and tumor metastasis. Methods: We used peripheral blood mononuclear cell (PBMC) samples from 520 patients with various tumor types and 109 healthy volunteers. The absolute numbers of lymphocytes and monocytes were identified by an automated blood analyzer, and multi-parameter flow cytometry was used to examine the subsets of natural killer (NK) cells (CD3-CD16+CD56+), T cells (CD3+CD4+/CD8+), and mononuclear cells (CD14+) in PBMC. Results: The percentage of T cells (CD3+) in peripheral blood mononuclear cells (PBMC) was 55.83% VS 45.54% (P<0.0001) between healthy volunteers and tumor patients, showing a significant downward trend. Meanwhile, the percentages of monocytes (CD14+) and NK cells (CD3-CD16+CD56+) showed a significant upward trend. Single factor or multifactor analysis yielded identical findings on the proportion of PBMC between healthy individuals and patients with different malignancies, considering the three confounding variables of age, sex, and tumor metastasis. Conclusion: The proportion and absolute counting of acquired immune T cells, innate immune NK cells, and monocytes in PBMCs all exhibit substantial changes between cancer patients and healthy individuals, and the differences are influenced by age, sex, and tumor progression.
摘要：
背景：为了更好地评估外周免疫状态，帮助肿瘤的早期诊断和预后，我们比较了健康个体和不同年龄肿瘤患者外周血免疫细胞亚群的比例和绝对计数，考虑性别和肿瘤转移的影响。方法：我们使用了520例不同类型肿瘤患者和109例健康志愿者的外周血单个核细胞（PBMC）样本。通过自动血液分析仪鉴定淋巴细胞和单核细胞的绝对数量，多参数流式细胞术用于检测自然杀伤（NK）细胞亚群（CD3-CD16+CD56+），T细胞（CD3+CD4+/CD8+），和PBMC中的单核细胞(CD14+)。结果：健康志愿者和肿瘤患者外周血单个核细胞（PBMC）中T细胞（CD3）的百分比为55.83％vs45.54％（P<0.0001），显着下降趋势。同时,单核细胞(CD14+)和NK细胞(CD3-CD16+CD56+)百分比呈显著上升趋势。单因素或多因素分析对健康个体和不同恶性肿瘤患者的PBMC比例产生了相同的发现。考虑到年龄的三个混杂变量，性别,和肿瘤转移。结论:获得性免疫T细胞的比例和绝对计数,先天免疫NK细胞，PBMC中的单核细胞在癌症患者和健康个体之间都表现出实质性的变化，差异受年龄的影响，性别,和肿瘤进展。
