PDF(Pubmed)
Abstract：
UNASSIGNED: Klebsiella pneumoniae is one of the most common causes of clinical and asymptomatic mastitis in dairy cattle, as well as in milk and dairy products that affect milk quality. Mastitis caused by K. pneumoniae is even more serious due to its poor response to antibiotic therapy. The aim of this study was to detect and identify the presence of K. pneumoniae in milk and dairy products produced in Libya.
UNASSIGNED: A total of 234 samples were randomly collected from various locations in Libya. Samples were examined for the presence of K. pneumoniae using conventional cultural techniques, including cultivation in violet red bile agar plus 4-methylumbelliferyl-ß-D-glucuronide broth and CHROM agar, followed by polymerase chain reaction identification and partial sequencing of 16S rRNA.
UNASSIGNED: Of the 234 samples of milk and dairy products collected, 16 (6.8%) isolates revealed mucoid colonies on agar media that were phenotypically suggested to be K. pneumoniae. Identification of isolates was confirmed using molecular techniques (16S rRNA). Among the examined samples, K. pneumoniae was recovered from camel\'s milk, raw cow\'s milk, raw fermented milk, Maasora cheese, Ricotta cheese, soft cheese, full cream milk powder, milk powder infant formula, cereal baby food, and growing-up formula. Antibiotic susceptibility testing was performed on 12 of the 16 K. pneumoniae isolates, and the results showed that K. pneumoniae isolates were resistant to more than eight antibiotics; interestingly, two isolates showed metallo-beta-lactamase (MBL) production.
UNASSIGNED: K. pneumoniae is considered a risk to human health because many of these products do not comply with the microbiological criteria of international and/or Libyan standards. This study emphasized the relationship between K. pneumoniae and raw milk, cheese, milk powder, and infant milk retailed in Libya. There is a need to take the necessary measures to ensure effective hygiene practices during production in dairy factories, handling, and distribution on the market, in particular at a small local production scale.
UNASSIGNED: A total of 234 samples were randomly collected from various locations in Libya. Samples were examined for the presence of K. pneumoniae using conventional cultural techniques, including cultivation in violet red bile agar plus 4-methylumbelliferyl-ß-D-glucuronide broth and CHROM agar, followed by polymerase chain reaction identification and partial sequencing of 16S rRNA.
UNASSIGNED: Of the 234 samples of milk and dairy products collected, 16 (6.8%) isolates revealed mucoid colonies on agar media that were phenotypically suggested to be K. pneumoniae. Identification of isolates was confirmed using molecular techniques (16S rRNA). Among the examined samples, K. pneumoniae was recovered from camel\'s milk, raw cow\'s milk, raw fermented milk, Maasora cheese, Ricotta cheese, soft cheese, full cream milk powder, milk powder infant formula, cereal baby food, and growing-up formula. Antibiotic susceptibility testing was performed on 12 of the 16 K. pneumoniae isolates, and the results showed that K. pneumoniae isolates were resistant to more than eight antibiotics; interestingly, two isolates showed metallo-beta-lactamase (MBL) production.
UNASSIGNED: K. pneumoniae is considered a risk to human health because many of these products do not comply with the microbiological criteria of international and/or Libyan standards. This study emphasized the relationship between K. pneumoniae and raw milk, cheese, milk powder, and infant milk retailed in Libya. There is a need to take the necessary measures to ensure effective hygiene practices during production in dairy factories, handling, and distribution on the market, in particular at a small local production scale.
摘要：
■肺炎克雷伯菌是奶牛临床和无症状性乳腺炎的最常见原因之一，以及影响牛奶质量的牛奶和乳制品。由肺炎克雷伯菌引起的乳腺炎由于其对抗生素治疗的不良反应而甚至更为严重。这项研究的目的是检测和鉴定在利比亚生产的牛奶和乳制品中肺炎克雷伯菌的存在。
■从利比亚的不同地点随机收集了总共234个样本。使用常规培养技术检查样品中肺炎克雷伯菌的存在，包括在紫红色胆汁琼脂加4-甲基伞形酮-β-D-葡糖苷酸肉汤和CHROM琼脂中培养，随后进行聚合酶链反应鉴定和16SrRNA部分测序。
■在收集的234份牛奶和乳制品样品中，16个（6.8％）的分离株在琼脂培养基上显示出粘液样菌落，在表型上被认为是肺炎克雷伯菌。使用分子技术(16SrRNA)确认分离物的鉴定。在检查的样本中，肺炎克雷伯菌是从骆驼奶中回收的，生牛奶，生发酵乳，Maasora奶酪,意大利乳清干酪,软奶酪,全脂奶粉,婴儿配方奶粉,谷类婴儿食品,和成长公式。对16个肺炎克雷伯菌分离株中的12个进行了抗生素敏感性测试，结果显示肺炎克雷伯菌分离株对八种以上抗生素有耐药性；有趣的是，两个分离物显示产生金属β-内酰胺酶(MBL).
■K.肺炎被认为是对人类健康的危险，因为许多这些产品不符合国际和/或利比亚标准的微生物学标准。这项研究强调了肺炎克雷伯菌与原料乳之间的关系，奶酪,奶粉,和在利比亚零售的婴儿牛奶。有必要采取必要措施，以确保乳品厂生产期间的有效卫生习惯，处理,以及在市场上的分销，特别是在当地生产规模很小的情况下。
■从利比亚的不同地点随机收集了总共234个样本。使用常规培养技术检查样品中肺炎克雷伯菌的存在，包括在紫红色胆汁琼脂加4-甲基伞形酮-β-D-葡糖苷酸肉汤和CHROM琼脂中培养，随后进行聚合酶链反应鉴定和16SrRNA部分测序。
■在收集的234份牛奶和乳制品样品中，16个（6.8％）的分离株在琼脂培养基上显示出粘液样菌落，在表型上被认为是肺炎克雷伯菌。使用分子技术(16SrRNA)确认分离物的鉴定。在检查的样本中，肺炎克雷伯菌是从骆驼奶中回收的，生牛奶，生发酵乳，Maasora奶酪,意大利乳清干酪,软奶酪,全脂奶粉,婴儿配方奶粉,谷类婴儿食品,和成长公式。对16个肺炎克雷伯菌分离株中的12个进行了抗生素敏感性测试，结果显示肺炎克雷伯菌分离株对八种以上抗生素有耐药性；有趣的是，两个分离物显示产生金属β-内酰胺酶(MBL).
■K.肺炎被认为是对人类健康的危险，因为许多这些产品不符合国际和/或利比亚标准的微生物学标准。这项研究强调了肺炎克雷伯菌与原料乳之间的关系，奶酪,奶粉,和在利比亚零售的婴儿牛奶。有必要采取必要措施，以确保乳品厂生产期间的有效卫生习惯，处理,以及在市场上的分销，特别是在当地生产规模很小的情况下。
