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Abstract：
UNASSIGNED: Systemic lupus erythematosus (SLE) is a diffuse connective tissue disease with complex clinical manifestations and prolonged course. The early diagnosis and condition monitoring of SLE are crucial to disease prognosis.
UNASSIGNED: To assess the diagnostic value of long noncoding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) in childhood-onset SLE (cSLE).
UNASSIGNED: Fifty-seven children diagnosed with SLE, 40 children diagnosed with juvenile idiopathic arthritis (JIA), and 40 healthy children were included. Peripheral blood samples from each patient were collected. A quantitative polymerase chain reaction was used to confirm the expression of lncNEAT1_1 and lncNEAT1_2 in peripheral blood. Associations among parameters were analyzed using the Mann-Whitney U test or independent sample t-test.
UNASSIGNED: The expression of both lncNEAT1_1 and lncNEAT1_2 in patients with cSLE were significantly higher than that of healthy control and patients with JIA. Receiver operating characteristic curves revealed an area under the curve (AUC) of 0.633 (95% confidence interval [CI], 0.524-0.742; P = 0.024) for lncNEAT1_1. The AUC of lncNEAT1_2 was 0.812 (95% CI, 0.727-0.897; P < 0.0001) to discriminate individuals with cSLE from health control and children with JIA with a sensitivity of 0.622 and a specificity of 0.925. Moreover, lncNEAT1_2 expression was higher in patients with cSLE presenting with fever, lupus nephritis, elevated erythrocyte sedimentation rate, active disease activity, and decreased C3 level, compared with those without these conditions. However, no similar correlation was observed for lncNEAT1_1.
UNASSIGNED: The expression of lncNEAT1_2 was significantly elevated in children with SLE, especially those with fever, renal involvement, and low C3 levels. These findings suggest that lncNEAT1_2 may represent a potential biomarker for cSLE.
UNASSIGNED: To assess the diagnostic value of long noncoding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) in childhood-onset SLE (cSLE).
UNASSIGNED: Fifty-seven children diagnosed with SLE, 40 children diagnosed with juvenile idiopathic arthritis (JIA), and 40 healthy children were included. Peripheral blood samples from each patient were collected. A quantitative polymerase chain reaction was used to confirm the expression of lncNEAT1_1 and lncNEAT1_2 in peripheral blood. Associations among parameters were analyzed using the Mann-Whitney U test or independent sample t-test.
UNASSIGNED: The expression of both lncNEAT1_1 and lncNEAT1_2 in patients with cSLE were significantly higher than that of healthy control and patients with JIA. Receiver operating characteristic curves revealed an area under the curve (AUC) of 0.633 (95% confidence interval [CI], 0.524-0.742; P = 0.024) for lncNEAT1_1. The AUC of lncNEAT1_2 was 0.812 (95% CI, 0.727-0.897; P < 0.0001) to discriminate individuals with cSLE from health control and children with JIA with a sensitivity of 0.622 and a specificity of 0.925. Moreover, lncNEAT1_2 expression was higher in patients with cSLE presenting with fever, lupus nephritis, elevated erythrocyte sedimentation rate, active disease activity, and decreased C3 level, compared with those without these conditions. However, no similar correlation was observed for lncNEAT1_1.
UNASSIGNED: The expression of lncNEAT1_2 was significantly elevated in children with SLE, especially those with fever, renal involvement, and low C3 levels. These findings suggest that lncNEAT1_2 may represent a potential biomarker for cSLE.
摘要：
■系统性红斑狼疮（SLE）是一种弥漫性结缔组织疾病，临床表现复杂，病程延长。SLE的早期诊断和病情监测对疾病预后至关重要。
■评估长链非编码RNA（lncRNA）核富集丰富转录物1（NEAT1）在儿童发作性SLE（cSLE）中的诊断价值。
■57名被诊断为SLE的儿童，40名被诊断患有幼年特发性关节炎（JIA）的儿童，包括40名健康儿童。收集来自每个患者的外周血样品。定量聚合酶链反应用于证实lncNEAT1_1和lncNEAT1_2在外周血中的表达。使用Mann-WhitneyU检验或独立样本t检验分析参数之间的关联。
■lncNEAT1_1和lncNEAT1_2在cSLE患者中的表达均明显高于健康对照组和JIA患者。受试者工作特征曲线显示曲线下面积（AUC）为0.633（95％置信区间[CI]，lncNEAT1_1的0.524-0.742；P=0.024）。lncNEAT1_2的AUC为0.812（95％CI，0.727-0.897；P<0.0001），用于区分cSLE个体与健康对照和JIA儿童的敏感性为0.622，特异性为0.925。此外，lncNEAT1_2在表现为发热的cSLE患者中表达较高，狼疮性肾炎,红细胞沉降率升高，活跃的疾病活动，C3水平下降，与没有这些条件的人相比。然而,lncNEAT1_1没有观察到类似的相关性。
■lncNEAT1_2的表达在SLE患儿中显著升高,尤其是那些发烧的人，肾受累,和低C3水平。这些发现表明lncNEAT1_2可能代表cSLE的潜在生物标志物。
■评估长链非编码RNA（lncRNA）核富集丰富转录物1（NEAT1）在儿童发作性SLE（cSLE）中的诊断价值。
■57名被诊断为SLE的儿童，40名被诊断患有幼年特发性关节炎（JIA）的儿童，包括40名健康儿童。收集来自每个患者的外周血样品。定量聚合酶链反应用于证实lncNEAT1_1和lncNEAT1_2在外周血中的表达。使用Mann-WhitneyU检验或独立样本t检验分析参数之间的关联。
■lncNEAT1_1和lncNEAT1_2在cSLE患者中的表达均明显高于健康对照组和JIA患者。受试者工作特征曲线显示曲线下面积（AUC）为0.633（95％置信区间[CI]，lncNEAT1_1的0.524-0.742；P=0.024）。lncNEAT1_2的AUC为0.812（95％CI，0.727-0.897；P<0.0001），用于区分cSLE个体与健康对照和JIA儿童的敏感性为0.622，特异性为0.925。此外，lncNEAT1_2在表现为发热的cSLE患者中表达较高，狼疮性肾炎,红细胞沉降率升高，活跃的疾病活动，C3水平下降，与没有这些条件的人相比。然而,lncNEAT1_1没有观察到类似的相关性。
■lncNEAT1_2的表达在SLE患儿中显著升高,尤其是那些发烧的人，肾受累,和低C3水平。这些发现表明lncNEAT1_2可能代表cSLE的潜在生物标志物。
