PDF(Pubmed)
Abstract：
Vaginitis, a prevalent gynecological condition in women, is mainly caused by an imbalance in the vaginal micro-ecology. The two most common types of vaginitis are vaginal bacteriosis and vulvovaginal candidiasis, triggered by the virulent Gardnerella vaginalis and Candida albicans, respectively. In this study, a strain capable of inhibiting G. vaginalis and C. albicans was screened from vaginal secretions and identified as Lactobacillus gasseri based on 16S rRNA sequences. The strain, named L. gasseri VHProbi E09, could inhibit the growth of G. vaginalis and C. albicans under co-culture conditions by 99.07% ± 0.26% and 99.95% ± 0.01%, respectively. In addition, it could significantly inhibit the adhesion of these pathogens to vaginal epithelial cells. The strain further showed the ability to inhibit the enteropathogenic bacteria Escherichia coli and Salmonella enteritidis, to tolerate artificial gastric and intestinal fluids and to adhere to intestinal Caco-2 cells. These results suggest that L. gasseri VHProbi E09 holds promise for clinical trials and animal studies whether administered orally or directly into the vagina. Whole-genome analysis also revealed a genome consisting of 1752 genes for L. gasseri VHProbi E09, with subsequent analyses identifying seven genes related to adhesion and three genes related to bacteriocins. These adhesion- and bacteriocin-related genes provide a theoretical basis for understanding the mechanism of bacterial inhibition of the strain. The research conducted in this study suggests that L. gasseri VHProbi E09 may be considered as a potential probiotic, and further research can delve deeper into its efficacy as an agent which can restore a healthy vaginal ecosystem.
摘要：
阴道炎,女性普遍存在的妇科疾病，主要是由阴道微生态失衡引起的。最常见的两种阴道炎是阴道菌病和外阴阴道念珠菌病,由致命的阴道加德纳菌和白色念珠菌引发，分别。在这项研究中,从阴道分泌物中筛选能够抑制阴道芽孢杆菌和白色念珠菌的菌株，并基于16SrRNA序列鉴定为gasseri乳杆菌。应变，命名为L.gasseriVHProbiE09，在共培养条件下，可以抑制99.07％±0.26％和99.95％±0.01％的阴道和白色念珠菌的生长，分别。此外,它能显著抑制这些病原体对阴道上皮细胞的粘附。该菌株进一步显示出抑制肠致病菌大肠杆菌和肠炎沙门氏菌的能力，耐受人工胃液和肠液，并粘附于肠道Caco-2细胞。这些结果表明L.gasseriVHProbiE09具有临床试验和动物研究的前景，无论是口服还是直接进入阴道。全基因组分析还揭示了一个由1752个基因组成的基因组，用于L.gasseriVHProbiE09，随后的分析鉴定了7个与粘附相关的基因和3个与细菌素相关的基因。这些粘附和细菌素相关基因为了解该菌株的细菌抑制机制提供了理论基础。这项研究表明，L.gasseriVHProbiE09可能被认为是一种潜在的益生菌，进一步的研究可以更深入地研究其作为一种可以恢复健康阴道生态系统的药物的功效。
