Abstract：
BACKGROUND: Preeclampsia is a significant cause of maternal and fetal morbidity and mortality, particularly in low- and middle-income countries like South Africa.
OBJECTIVE: The aim of our study was to investigate the association between placental growth factor (PlGF) and soluble FMS-like tyrosine kinase-1 (sFlt-1) in South African preeclamptic women of African ancestry, comorbid with HIV infection.
METHODS: The study population consisted of women attending a regional hospital in Durban, South Africa, stratified by pregnancy type (normotensive pregnant and preeclampsia) and HIV status. Preeclampsia was defined as new-onset hypertension and proteinuria. DNA was obtained from whole blood. The SNPs of interest were rs722503 in sFlt-1 and rs4903273 in PlGF.
RESULTS: Our findings suggest that single nucleotide polymorphisms of rs722503 analysis show no significant associations between the genotypic frequencies of rs722503 variants and preeclampsia risk in either HIV-negative or HIV-positive groups of women of African ancestry. Similarly, the rs493273 polymorphism showed no significant association with preeclampsia risk in either HIV-negative or HIV-positive pregnant women. Additionally, comparisons of dominant, recessive, and over-dominant allele models did not reveal significant associations. These findings suggest that these genetic variants may not significantly contribute to preeclampsia development in this African ancestry population. However, significant differences were observed in the rs4903273 genotype frequencies between normotensive and preeclamptic women, regardless of HIV status, over dominant alleles AA + GG vs AG showed a significant difference [OR = 2.706; 95% Cl (1.199-5.979); adjusted p = 0.0234*], also in normotensive compared to EOPE (OR = 2.804; 95% Cl (1.151-6.89) p = 0.0326* and LOPE (OR = 2.601; 95% Cl (1.0310-6.539) p = 0.0492*), suggesting that they may be the potential role of this variant in preeclampsia susceptibility.
CONCLUSIONS: The findings suggest that the rs722503 and rs493273 polymorphisms do not significantly contribute to preeclampsia susceptibility in HIV-negative or HIV-positive pregnant women. However, the rs4903273 genotype frequencies showed notable differences between normotensive and preeclamptic women, indicating a potential association with preeclampsia development in the African ancestry population irrespective of HIV status.
OBJECTIVE: The aim of our study was to investigate the association between placental growth factor (PlGF) and soluble FMS-like tyrosine kinase-1 (sFlt-1) in South African preeclamptic women of African ancestry, comorbid with HIV infection.
METHODS: The study population consisted of women attending a regional hospital in Durban, South Africa, stratified by pregnancy type (normotensive pregnant and preeclampsia) and HIV status. Preeclampsia was defined as new-onset hypertension and proteinuria. DNA was obtained from whole blood. The SNPs of interest were rs722503 in sFlt-1 and rs4903273 in PlGF.
RESULTS: Our findings suggest that single nucleotide polymorphisms of rs722503 analysis show no significant associations between the genotypic frequencies of rs722503 variants and preeclampsia risk in either HIV-negative or HIV-positive groups of women of African ancestry. Similarly, the rs493273 polymorphism showed no significant association with preeclampsia risk in either HIV-negative or HIV-positive pregnant women. Additionally, comparisons of dominant, recessive, and over-dominant allele models did not reveal significant associations. These findings suggest that these genetic variants may not significantly contribute to preeclampsia development in this African ancestry population. However, significant differences were observed in the rs4903273 genotype frequencies between normotensive and preeclamptic women, regardless of HIV status, over dominant alleles AA + GG vs AG showed a significant difference [OR = 2.706; 95% Cl (1.199-5.979); adjusted p = 0.0234*], also in normotensive compared to EOPE (OR = 2.804; 95% Cl (1.151-6.89) p = 0.0326* and LOPE (OR = 2.601; 95% Cl (1.0310-6.539) p = 0.0492*), suggesting that they may be the potential role of this variant in preeclampsia susceptibility.
CONCLUSIONS: The findings suggest that the rs722503 and rs493273 polymorphisms do not significantly contribute to preeclampsia susceptibility in HIV-negative or HIV-positive pregnant women. However, the rs4903273 genotype frequencies showed notable differences between normotensive and preeclamptic women, indicating a potential association with preeclampsia development in the African ancestry population irrespective of HIV status.
摘要：
背景：子痫前期是孕产妇和胎儿发病率和死亡率的重要原因，特别是在南非等低收入和中等收入国家。
目的：我们的研究目的是调查胎盘生长因子（PlGF）和可溶性FMS样酪氨酸激酶-1（sFlt-1）在南非先兆子痫妇女非洲血统，与艾滋病毒感染共病。
方法：研究人群包括在德班地区医院就诊的女性，南非,按妊娠类型（正常妊娠和先兆子痫）和HIV状况分层。先兆子痫被定义为新发高血压和蛋白尿。从全血获得DNA。感兴趣的SNP是sFlt-1中的rs722503和PlGF中的rs4903273。
结果：我们的研究结果表明，rs722503的单核苷酸多态性分析显示，在非洲血统的HIV阴性或HIV阳性妇女群体中，rs722503变异的基因型频率与先兆子痫风险之间没有显著关联。同样，在HIV阴性或HIV阳性孕妇中,rs493273多态性与子痫前期风险均无显著关联.此外,占主导地位的比较，隐性,和过显性等位基因模型未显示显著关联。这些发现表明，这些遗传变异可能不会显着促进该非洲血统人群中先兆子痫的发展。然而,在正常血压和先兆子痫妇女之间的rs4903273基因型频率中观察到显着差异，无论艾滋病毒的状况如何，过显性等位基因AA+GG与AG显示显著差异[OR=2.706;95%Cl(1.199-5.979);调整后p=0.0234*],与EOPE相比，血压也正常（OR=2.804；95％Cl（1.151-6.89）p=0.0326*和LOPE（OR=2.601；95％Cl（1.0310-6.539）p=0.0492*），这表明它们可能是这种变异在先兆子痫易感性中的潜在作用。
结论：研究结果表明，rs722503和rs493273多态性对HIV阴性或HIV阳性孕妇的先兆子痫易感性没有显著影响。然而,rs4903273基因型频率在血压正常和先兆子痫妇女之间显示出显着差异，表明在非洲祖先人群中，无论HIV状况如何，都可能与先兆子痫的发展有关。
目的：我们的研究目的是调查胎盘生长因子（PlGF）和可溶性FMS样酪氨酸激酶-1（sFlt-1）在南非先兆子痫妇女非洲血统，与艾滋病毒感染共病。
方法：研究人群包括在德班地区医院就诊的女性，南非,按妊娠类型（正常妊娠和先兆子痫）和HIV状况分层。先兆子痫被定义为新发高血压和蛋白尿。从全血获得DNA。感兴趣的SNP是sFlt-1中的rs722503和PlGF中的rs4903273。
结果：我们的研究结果表明，rs722503的单核苷酸多态性分析显示，在非洲血统的HIV阴性或HIV阳性妇女群体中，rs722503变异的基因型频率与先兆子痫风险之间没有显著关联。同样，在HIV阴性或HIV阳性孕妇中,rs493273多态性与子痫前期风险均无显著关联.此外,占主导地位的比较，隐性,和过显性等位基因模型未显示显著关联。这些发现表明，这些遗传变异可能不会显着促进该非洲血统人群中先兆子痫的发展。然而,在正常血压和先兆子痫妇女之间的rs4903273基因型频率中观察到显着差异，无论艾滋病毒的状况如何，过显性等位基因AA+GG与AG显示显著差异[OR=2.706;95%Cl(1.199-5.979);调整后p=0.0234*],与EOPE相比，血压也正常（OR=2.804；95％Cl（1.151-6.89）p=0.0326*和LOPE（OR=2.601；95％Cl（1.0310-6.539）p=0.0492*），这表明它们可能是这种变异在先兆子痫易感性中的潜在作用。
结论：研究结果表明，rs722503和rs493273多态性对HIV阴性或HIV阳性孕妇的先兆子痫易感性没有显著影响。然而,rs4903273基因型频率在血压正常和先兆子痫妇女之间显示出显着差异，表明在非洲祖先人群中，无论HIV状况如何，都可能与先兆子痫的发展有关。
