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Abstract：
The study presents a series of examples of magnetic nanoparticle systems designed for the diagnosis of viral diseases. In this interdisciplinary work, we describe one of the most comprehensive synthetic approaches for the preparation and functionalization of smart nanoparticle systems for rapid and effective RT-PCR diagnostics and isolation of viral RNA. Twelve different organic ligands and inorganic porous silica were used for surface functionalization of the Fe3O4 magnetic core to increase the number of active centres for efficient RNA binding from human swab samples. Different nanoparticle systems with common beads were characterized by HRTEM, SEM, FT-IR, XRD, XPS and magnetic measurements. We demonstrate the application of the fundamental models modified to fit the experimental zero-field cooling magnetization data. We discuss the influence of the nanoparticle shell parameters (morphology, thickness, ligands) on the overall magnetic performance of the systems. The prepared nanoparticles were tested for the isolation of viral RNA from tissue samples infected with hepatitis E virus-HEV and from biofluid samples of SARS-CoV-2 positive patients. The efficiency of RNA isolation was quantified by RT-qPCR method.
摘要：
该研究提供了一系列用于诊断病毒性疾病的磁性纳米颗粒系统的示例。在这项跨学科的工作中，我们描述了用于快速有效的RT-PCR诊断和分离病毒RNA的智能纳米颗粒系统的制备和功能化的最全面的合成方法之一。使用十二种不同的有机配体和无机多孔二氧化硅对Fe3O4磁芯进行表面官能化，以增加活性中心的数量，以有效结合人拭子样品中的RNA。通过HRTEM表征具有常见珠子的不同纳米颗粒系统，SEM,FT-IR,XRD,XPS和磁测量。我们演示了修改后的基本模型的应用，以拟合实验零场冷却磁化数据。我们讨论了纳米粒子壳参数的影响(形态，厚度,配体）对系统的整体磁性能的影响。测试制备的纳米颗粒从感染戊型肝炎病毒-HEV的组织样品和SARS-CoV-2阳性患者的生物流体样品中分离病毒RNA。通过RT-qPCR方法定量RNA分离的效率。
