Abstract：
Black soldier fly (BSF; Hermetia illucens) is a promising insect species for food and feed production as its larvae can convert different organic waste to high-value protein. Selective breeding is one way to optimize production, but the potential of breeding is only starting to be explored and not yet utilized for BSF. To assist in monitoring a captive population and implementing a breeding program, genomics tools are imperative. We conducted whole genome sequencing of two captive populations separated by geographical distance - Denmark (DK) and Texas, USA (TX). Various population genetics analyses revealed a moderate genetic differentiation between two populations. Moreover, we observed higher inbreeding in the DK population, and the detection of a subpopulation within DK population aligned well with the recent foundation of the DK population from two captive populations. Additionally, we generated gene ontology annotation and variants annotation for wider potential applications. Our findings establish a robust marker set for research in population genetics, facilitating the monitoring of inbreeding and laying the groundwork for practical breeding programs for BSF.
摘要：
黑兵蝇（BSF;Hermetiaillucens）是一种有前途的昆虫物种，用于食品和饲料生产，因为它的幼虫可以将不同的有机废物转化为高价值的蛋白质。选育是优化生产的途径之一,但是育种的潜力才刚刚开始探索，尚未用于BSF。协助监测圈养人口和实施育种计划，基因组学工具势在必行。我们对两个按地理距离分开的圈养种群进行了全基因组测序-丹麦（DK）和德克萨斯州，美国（TX）。各种种群遗传学分析显示，两个种群之间存在中等遗传差异。此外，我们观察到DK种群的近亲繁殖率较高，并且在DK种群中检测到一个亚群与两个圈养种群的DK种群的最新基础非常吻合。此外,我们生成了基因本体注释和变体注释，用于更广泛的潜在应用。我们的发现为群体遗传学研究建立了一个强大的标记集，促进近亲繁殖的监测，并为BSF的实际育种计划奠定基础。
