Abstract：
CD147 is a T cell activation-associated molecule which is closely involved in the formation of the immune synapse (IS). However, the precise role of CD147 in T cell activation and IS formation remains unclear. In the present study, we demonstrated that CD147 translocated to the IS upon T cell activation and was primarily distributed in the peripheral super molecular cluster (p-SMAC). The knock down of CD147 expression in T cells, but not in B cells, impaired IS formation. CD147 participated in IS formation between T cells and different types of antigen-presenting cells (APCs), including macrophages and dendritic cells. Ligation of CD147 with its monoclonal antibody (mAb) HAb18 effectively inhibited T cell activation and IL-2 secretion. CD98, a critical molecule interacting with CD147, was distributed in IS in a CD147-dependent way. Phosphorylation levels of T cell receptor (TCR) related molecules, like ZAP-70, ERK, and cJun, were down-regulated by CD147 ligation, which is crucial for the interaction of CD147 and TCR signaling transduction. CD147 is indispensable for the formation of immune synapses and plays an important role in the regulation of its function.
摘要：
CD147是一种T细胞活化相关分子，与免疫突触（IS）的形成密切相关。然而,CD147在T细胞活化和IS形成中的确切作用尚不清楚.在本研究中,我们证明了CD147在T细胞激活后易位到IS，并主要分布在外周超分子簇（p-SMAC）中。T细胞中CD147表达的敲低，但不是在B细胞中，受损IS形成。CD147参与了T细胞和不同类型的抗原呈递细胞（APC）之间的IS形成，包括巨噬细胞和树突状细胞。CD147与其单克隆抗体（mAb）HAb18连接可有效抑制T细胞活化和IL-2分泌。CD98是与CD147相互作用的关键分子，以CD147依赖性方式分布在IS中。T细胞受体（TCR）相关分子的磷酸化水平，比如ZAP-70ERK,还有cJun,通过CD147连接下调，这对于CD147和TCR信号转导的相互作用至关重要。CD147对于免疫突触的形成是必不可少的，在其功能的调节中起着重要作用。
