Abstract：
Proteases, enzymes that hydrolyze peptide bonds, have various applications in medicine, clinical applications, and pharmaceutical development. They are used in cancer treatment, wound debridement, contact lens cleaning, prion degradation, biofilm removal, and fibrinolytic agents. Proteases are also crucial in cardiovascular disease treatment, emphasizing the need for safe, affordable, and effective fibrinolytic drugs. Proteolytic enzymes and protease biosensors are increasingly used in diagnostic and therapeutic applications. Advanced technologies, such as nanomaterials-based sensors, are being developed to enhance the sensitivity, specificity, and versatility of protease biosensors. These biosensors are becoming effective tools for disease detection due to their precision and rapidity. They can detect extracellular and intracellular proteases, as well as fluorescence-based methods for real-time and label-free detection of virus-related proteases. The active utilization of proteolytic enzymatic biosensors is expected to expand significantly in biomedical research, in-vitro model systems, and drug development. We focused on journal articles and books published in English between 1982 and 2024 for this study.
摘要：
蛋白酶,水解肽键的酶，在医学上有各种应用，临床应用，和药物开发。它们用于癌症治疗，伤口清创术,隐形眼镜清洁，朊病毒降解，生物膜去除，和纤维蛋白溶解剂。蛋白酶在心血管疾病治疗中也至关重要，强调安全的必要性，负担得起的,和有效的纤维蛋白溶解药物。蛋白水解酶和蛋白酶生物传感器越来越多地用于诊断和治疗应用。先进的技术,例如基于纳米材料的传感器，正在开发以增强灵敏度，特异性，和蛋白酶生物传感器的多功能性。这些生物传感器由于其精确性和快速性而成为疾病检测的有效工具。它们可以检测细胞外和细胞内的蛋白酶，以及基于荧光的实时和无标记检测病毒相关蛋白酶的方法。蛋白水解酶生物传感器的积极利用有望在生物医学研究中得到显着扩展。体外模型系统，和药物开发。本研究的重点是1982年至2024年间以英文发表的期刊文章和书籍。
