PDF(Pubmed)
Abstract：
BACKGROUND: Cucurbita pepo cv Dayangua (CPD) is an edible plant with diverse pharmacological properties. The current research on CPD has primarily focused on initial investigations of its chemical composition and pharmacological effects, and no comprehensive toxicity assessment has been conducted to date.
METHODS: In the present study, the toxicity of CPD was evaluated through both acute and sub-chronic oral toxicity tests in mice. 16S rDNA sequencing was used to analyze the composition of the gut microbiota of mice at different time points to observe the effect of CPD on these microbial communities.
RESULTS: In the acute toxicity test, CPD exhibited low toxicity, with a median lethal dose (LD50) > 2000 mg/kg. The sub-chronic toxicity test indicated that CPD administration at doses of 200, 400, and 600 mg/kg did not cause mortality or significant organ damage in mice. Furthermore, analysis of the gut microbiota after gavage administration of CPD at 400 and 600 mg/kg revealed an improved abundance of some beneficial gut bacteria.
CONCLUSIONS: In summary, no acute or sub-chronic toxic effects were observed in mice following the oral administration of CPD. CPD did not affect the structure and diversity of the gut microbiota and may contribute to an increase in the number of beneficial gut bacteria.
METHODS: In the present study, the toxicity of CPD was evaluated through both acute and sub-chronic oral toxicity tests in mice. 16S rDNA sequencing was used to analyze the composition of the gut microbiota of mice at different time points to observe the effect of CPD on these microbial communities.
RESULTS: In the acute toxicity test, CPD exhibited low toxicity, with a median lethal dose (LD50) > 2000 mg/kg. The sub-chronic toxicity test indicated that CPD administration at doses of 200, 400, and 600 mg/kg did not cause mortality or significant organ damage in mice. Furthermore, analysis of the gut microbiota after gavage administration of CPD at 400 and 600 mg/kg revealed an improved abundance of some beneficial gut bacteria.
CONCLUSIONS: In summary, no acute or sub-chronic toxic effects were observed in mice following the oral administration of CPD. CPD did not affect the structure and diversity of the gut microbiota and may contribute to an increase in the number of beneficial gut bacteria.
摘要：
背景：西葫芦（CPD）是一种具有多种药理特性的可食用植物。目前对CPD的研究主要集中在对其化学成分和药理作用的初步研究上，迄今为止，尚未进行全面的毒性评估。
方法：在本研究中，通过小鼠急性和亚慢性口服毒性试验评估CPD的毒性.16SrDNA测序用于分析小鼠在不同时间点的肠道菌群组成，以观察CPD对这些微生物群落的影响。
结果：在急性毒性试验中，CPD表现出低毒性，中位致死剂量（LD50）>2000mg/kg。亚慢性毒性试验表明，以200、400和600mg/kg的剂量施用CPD不会导致小鼠死亡或明显的器官损伤。此外，在以400和600mg/kg的浓度灌胃施用CPD后,对肠道微生物群的分析显示,一些有益的肠道细菌的丰度提高。
结论：总之，口服CPD后，小鼠未观察到急性或亚慢性毒性作用。CPD不会影响肠道微生物群的结构和多样性，并且可能有助于增加有益肠道细菌的数量。
方法：在本研究中，通过小鼠急性和亚慢性口服毒性试验评估CPD的毒性.16SrDNA测序用于分析小鼠在不同时间点的肠道菌群组成，以观察CPD对这些微生物群落的影响。
结果：在急性毒性试验中，CPD表现出低毒性，中位致死剂量（LD50）>2000mg/kg。亚慢性毒性试验表明，以200、400和600mg/kg的剂量施用CPD不会导致小鼠死亡或明显的器官损伤。此外，在以400和600mg/kg的浓度灌胃施用CPD后,对肠道微生物群的分析显示,一些有益的肠道细菌的丰度提高。
结论：总之，口服CPD后，小鼠未观察到急性或亚慢性毒性作用。CPD不会影响肠道微生物群的结构和多样性，并且可能有助于增加有益肠道细菌的数量。
