PDF(Pubmed)
Abstract：
Dicytostelium firmibasis is a member of Dictyostelia, a group of social amoebae that upon starvation display aggregative multicellularity where the amoebae transition from uni- to multicellular life. The D. firmibasis genome assembly that is currently available is of limited use due to its low contiguity, large number of undetermined bases, and lack of annotations. Here we used Nanopore long read sequencing, complemented with Illumina sequencing, and developmental transcriptomics as well as small RNA-sequencing, to present a new, fully annotated, chromosome-level D. firmibasis genome assembly. The new assembly contains no undetermined bases, and consists mainly of six large contigs representing the chromosomes, as well as a complete mitochondrial genome. This new genome assembly will be a valuable tool, allowing comprehensive comparison to Dictyostelium discoideum, the dictyostelid genetically tractable model. Further, the new genome will be important for studies of evolutionary processes governing the transition from unicellular to multicellular organisms and will aid in the sequencing and annotation of other dictyostelids genomes, many of which are currently of poor quality.
摘要：
二重菌是木纹菌的成员，一组社会变形虫，在饥饿时表现出聚集的多细胞性，变形虫从单细胞生命过渡到多细胞生命。目前可用的D.firmisis基因组组装由于其低连续性而用途有限，大量不确定的碱基，缺乏注释。在这里，我们使用了纳米孔长读数测序，辅以Illumina测序，和发育转录组学以及小RNA测序，呈现一个新的，完全注释,染色体水平D.firmisis基因组组装。新程序集不包含未确定的基数，主要由六个代表染色体的大重叠群组成，以及完整的线粒体基因组.这种新的基因组组装将是一个有价值的工具，允许与盘基网柄菌进行全面比较，双歧杆菌基因可处理的模型。Further,新的基因组将是重要的进化过程的研究控制从单细胞到多细胞生物的过渡，并将有助于测序和注释的其他二重性基因组，其中许多目前质量很差。
