PDF(Pubmed)
Abstract：
Heat shock protein 70 (HSP70), the most prominent and well-characterized stress protein in animals, plays an important role in assisting animals in responding to various adverse conditions. In the present study, a total of 113 HSP70 gene family members were identified in the updated genome of Magallana gigas (designated MgHSP70) (previously known as Crassostrea gigas). There were 75, 12, 11, and 8 HSP70s located in the cytoplasm, nucleus, mitochondria, and endoplasmic reticulum, respectively, and 7 HSP70s were located in both the nucleus and cytoplasm. Among 113 MgHSP70 genes, 107 were unevenly distributed in 8 chromosomes of M. gigas with the greatest number in chromosome 07 (61 genes, 57.01%). The MgHSP70 gene family members were mainly assigned into five clusters, among which the HSPa12 subfamily underwent lineage-specific expansion, consisting of 89 members. A total of 68 MgHSP70 genes (60.18%) were tandemly duplicated and formed 30 gene pairs, among which 14 gene pairs were under strong positive selection. In general, the expression of MgHSP70s was tissue-specific, with the highest expression in labial palp and gill and the lowest expression in adductor muscle and hemocytes. There were 35, 31, and 47 significantly upregulated genes at 6, 12, and 24 h after heat shock treatment (28 °C), respectively. The expression patterns of different tandemly duplicated genes exhibited distinct characteristics after shock treatment, indicating that these genes may have different functions. Nevertheless, genes within the same tandemly duplicated group exhibit similar expression patterns. Most of the tandemly duplicated HSP70 gene pairs showed the highest expression levels at 24 h. This study provides a comprehensive description of the MgHSP70 gene family in M. gigas and offers valuable insights into the functions of HSP70 in the mollusc adaptation of oysters to environmental stress.
摘要：
热休克蛋白70(HSP70),动物中最突出和特征最明确的应激蛋白，在帮助动物应对各种不利条件方面发挥着重要作用。在本研究中,在Magallanagigas（命名为MgHSP70）（以前称为Crassostreagigas）的更新基因组中，总共鉴定出113个HSP70基因家族成员。细胞质中有75、12、11和8个HSP70s，核，线粒体和内质网，分别,7个HSP70s位于细胞核和细胞质中。在113个MgHSP70基因中，107个不均匀地分布在M.gigas的8条染色体中，在07号染色体中数量最多（61个基因，57.01%）。MgHSP70基因家族成员主要分为五个簇，其中HSPa12亚家族经历了谱系特异性扩增，由89名成员组成。共有68个(60.18%)MgHSP70基因串联重复,形成30个基因对,其中14个基因对处于强阳性选择状态。总的来说,MgHSP70s的表达是组织特异性的，在唇触诊和g中表达最高，在内收肌和血细胞中表达最低。热休克处理(28℃)后6、12和24h分别有35、31和47个基因显著上调,分别。不同串联重复基因的表达模式在热胁迫后表现出不同的特征，表明这些基因可能具有不同的功能。然而，同一串联重复组中的基因表现出相似的表达模式。大多数串联重复的HSP70基因对在24h时显示出最高的表达水平。这项研究提供了M.gigas中MgHSP70基因家族的全面描述，并为HSP70在牡蛎对环境胁迫的软体动物适应中的功能提供了有价值的见解。
