Abstract：
There is the rapid growth in application of Brillouin scattering spectroscopy to biomedical objects in order to characterize their mechanoelastic properties in this way. However, the possibilities and limitations of the method when applied to tissues have not yet been clarified. Here, applicability of Brillouin spectroscopy for testing the elastic response of medically relevant tissues of bovine jugular vein and pericardium was considered. Parameters of the Brillouin peak were studied for samples untreated, diepoxide-fixed, and preserved after treatment in alcohol solutions. It was found that diepoxide cross-linking resulted to a slight tendency to increase the Brillouin position for hydrated tissues. The variations in the position and width of the Brillouin peaks, associated with local fluctuations in water concentration, were reduced after diepoxide treatment in the case of the pericardium, but not in the case of the vein wall. To obtain more information about the elastic response of the protein scaffold without the participation of water, dried samples were also studied. Brillouin spectra of the dried pericardium and vein wall revealed a significant increase in the Brillouin peak position (elastic modulus) after conservation in alcohol. In the case of the vein wall, this effect was found for both collagen and elastin-related peaks, which were identified in the Brillouin spectrum. This result corresponds to a denser packing of fibrous proteins after preservation in alcohol solutions. The ability of Brillouin spectroscopy to independently characterize the effect of treatment on the instantaneous elastic modulus of various tissue components is also attractive for its application in the development of new materials for bioimplants. A comparison of the Brillouin longitudinal and Young\'s elastic moduli determined for the hydrated samples of the vein and pericardium showed that there is no clear correspondence between these material parameters. The usefulness of using both experimental methods to obtain new information about the elastic response of the material is discussed.
摘要：
布里渊散射光谱在生物医学对象中的应用迅速发展，以便以这种方式表征其机械弹性。然而,该方法应用于组织的可能性和局限性尚未得到澄清.这里,考虑了布里渊光谱法用于测试牛颈静脉和心包的医学相关组织的弹性响应的适用性。对未经处理的样品研究了布里渊峰的参数，二环氧化物固定，在酒精溶液中处理后保存。发现二环氧化物交联导致水合组织的布里渊位置略有增加的趋势。布里渊峰的位置和宽度的变化，与水浓度的局部波动有关，在心包的情况下，二环氧化物治疗后减少，但不是在静脉壁的情况下。为了获得更多关于没有水参与的蛋白质支架的弹性响应的信息，干燥的样品也进行了研究。干燥的心包和静脉壁的布里渊光谱显示，在酒精中保存后，布里渊峰位置（弹性模量）显着增加。在静脉壁的情况下，胶原蛋白和弹性蛋白相关峰都有这种作用，在布里渊光谱中被鉴定出来。该结果对应于在醇溶液中保存后纤维状蛋白的更致密的堆积。布里渊光谱学独立表征治疗对各种组织成分的瞬时弹性模量的影响的能力对于其在生物植入物新材料的开发中的应用也具有吸引力。对静脉和心包的水合样品确定的布里渊纵向和杨氏弹性模量的比较表明，这些材料参数之间没有明确的对应关系。讨论了使用两种实验方法来获得有关材料弹性响应的新信息的有用性。
