Abstract：
Phenolics in bound form extensively exist in cereal dietary fiber, especially insoluble fiber, while their release profile in gastrointestinal tract and contribution to the potential positive effects of dietary fiber in modulating gut microbiota still needs to be disclosed. In this work, the composition of bound phenolics (BPs) in triticale insoluble dietary fiber (TIDF) was studied, and in vitro gastrointestinal digestion as well as colonic fermentation were performed to investigate BPs liberation and their role in regulating intestinal flora of TIDF. It turned out that most BPs were unaccessible in digestion but partly released continuously during fermentation. 16 s rRNA sequencing demonstrated that TIDF possessed prebiotic effects by promoting anti-inflammatory while inhibiting proinflammatory bacteria alongside boosting SCFAs production and antioxidative BPs contributed a lot to these effects. Results indicated that TIDF held capabilities to regulate intestinal flora and BPs were important functional components to the health benefits of cereal dietary fiber.
摘要：
结合形式的酚类物质广泛存在于谷物膳食纤维中，尤其是不溶性纤维，尽管它们在胃肠道中的释放曲线以及膳食纤维对调节肠道微生物群的潜在积极作用的贡献仍需要披露。在这项工作中,研究了黑麦不溶性膳食纤维(TIDF)中结合酚类物质(BPs)的组成,并进行了体外胃肠消化和结肠发酵，以研究BPs的释放及其在TIDF肠道菌群调节中的作用。事实证明，大多数BPs在消化中是无法获得的，但在发酵过程中部分连续释放。16srRNA测序表明，TIDF通过促进抗炎同时抑制促炎细菌以及促进SCFA产生和抗氧化BPs而具有益生元作用。结果表明，TIDF具有调节肠道菌群的能力，BPs是谷物膳食纤维健康益处的重要功能成分。
