Abstract：
Leukemia is an incurable disease; it exhibits strong resistance to chemotherapy and other therapies, and it represents the most common childhood cancer and mortality. The cytotoxic of amygdalin (AMG) against the cell line of human monocytic leukemia (THP-1) was recorded, before determining other pharmacological effects. The cells were exposed to AMG for 24 hr at 37°C at different concentrations, the cytotoxic effect was determined via the MTT assay. The cells and the supernatant were collected for analyzing the oxidant/antioxidant status, apoptotic markers, and anti-microbial activity. Results showed a marked anti-proliferative cytotoxic effect of AMG which is concentration and time-dependent, the lipid peroxidation content was significantly decreased while the total thiol was increased in the treated cell line, significant up-regulation of Caspase-3 (Cas-3) and Bcl-2-associated X protein (BAX) and down-regulation of B-cell lymphoma 2 (Bcl-2). Furthermore, The bacterial activity was detected via Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and Disc Diffusion assays, while the antifungal evaluation was done by the Minimum Fungicidal Concentration (MFC). Antimicrobial experiments revealed that AMG exerted potent, broad-spectrum antimicrobial effects toward a diversity of dangerously infecting pathogens. In conclusion; the prevailing research suggests that AMG is an effective anticarcinogenic and antimicrobial substance. The utilization of AMG subsequently in masks or wound dressings to prevent bacterial & fungal infections, including mucormycosis following COVID-19, as well as infections caused by penicillium and aspergillus, is a highly effective strategy in combating resistant microorganisms.
摘要：
白血病是一种无法治愈的疾病；它对化疗和其他疗法表现出强烈的抵抗力，它代表了最常见的儿童癌症和死亡率。记录了苦杏仁苷（AMG）对人单核细胞白血病（THP-1）细胞系的细胞毒性，在确定其他药理作用之前。将细胞在37°C下以不同浓度暴露于AMG24小时，通过MTT测定细胞毒性作用。收集细胞和上清液用于分析氧化剂/抗氧化剂状态,凋亡标志物，和抗微生物活性。结果表明，AMG具有明显的抗增殖细胞毒性作用，该作用是浓度和时间依赖性的，在处理的细胞系中，脂质过氧化含量显着降低，而总硫醇增加，显著上调Caspase-3(Cas-3)和Bcl-2相关X蛋白(BAX),下调B细胞淋巴瘤2(Bcl-2)。此外，通过最低抑菌浓度（MIC）检测细菌活性，最小杀菌浓度（MBC），和椎间盘扩散测定，而抗真菌评估是通过最低杀真菌浓度（MFC）进行的。抗菌实验表明，AMG发挥了强大的作用，对多种危险感染病原体的广谱抗微生物作用。总之，目前的研究表明，AMG是一种有效的抗癌和抗菌物质。随后在口罩或伤口敷料中使用AMG以防止细菌和真菌感染，包括COVID-19后的毛霉菌病，以及由青霉和曲霉引起的感染，是对抗抗性微生物的高效策略。
