Abstract：
Several studies have demonstrated the correlation between Doppler velocimetric parameters of testicular artery and semen quality in domestic species, but in felines data are scarce. This study aimed to correlate the Doppler velocimetry of the testicular artery with sperm kinetics and sperm defects, in sedated and non-sedated cats. Forty tomcats were divided into two groups: sedated (SG; n=20) with dexmedetomidine (10 µm/kg) and ketamine (12 mg/kg), and non-sedated (NSG; n=20). The animals were subjected to ultrasound Doppler velocimetry of the distal supratesticular and marginal region of the testicular artery and subsequently orchiectomized. Epididymal tail spermatozoa were recovered and analyzed using a CASA system for motility, and morphology took place. Animals of SG presented a significantly higher velocity in the marginal region of the cat\'s testicular artery [peak systolic velocity (PSV) 11.51 cm/s; end-diastolic velocity (EDV) 7.72 cm/s] compared to NSG (PSV 7.72 cm/s, P < 0.001; EDV 4.93 cm/s, P < 0.001). Sedated cats presented higher pulsatility and resistivity indexes than non-sedated cats. The supratesticular PSV of NSG was moderately correlated with major (rs = 0621; P < 0.001) and total sperm defects (rs = 0614; P < 0001). Doppler velocimetry was fairly correlated with minor, major, and total sperm defects. In conclusion, Doppler velocimetric evaluation emerges as an important possibility in the reproductive evaluation of tomcats, once the testicular artery hemodynamics were associated with sperm defects. However, it is advisable to carry out this evaluation in non-sedated animals. If sedation is necessary, peripheral vasoconstriction should be considered.
摘要：
多项研究表明，家畜睾丸动脉多普勒测速参数与精液质量之间存在相关性，但是猫科动物的数据很少。这项研究旨在将睾丸动脉的多普勒测速与精子动力学和精子缺陷相关联，镇静剂和非镇静剂的猫。40只Tomcat被分为两组：使用右美托咪定（10µm/kg）和氯胺酮（12mg/kg）镇静（SG;n=20），和非镇静剂(NSG；n=20)。对动物进行睾丸动脉远端睾丸上和边缘区域的超声多普勒测速，然后切除睾丸。回收附睾尾精子，并使用CASA系统进行运动性分析，形态发生了。与NSG(PSV7.72cm/s,P<0.001;EDV4.93cm/s,P<0.001)。镇静猫比非镇静猫表现出更高的搏动性和电阻率指数。NSG的睾丸上PSV与主要（rs=0621；P<0.001）和总精子缺陷（rs=0614；P<0001）中度相关。多普勒测速与未成年人相当相关，major,和精子总缺陷。总之,多普勒测速评估已成为对猫进行生殖评估的重要可能性，一旦睾丸动脉血流动力学与精子缺陷有关。然而,建议在非镇静剂动物中进行此评估。如果需要镇静,应考虑外周血管收缩.
