Abstract：
We present a powerful method for direct mRNA detection based on ligation-based recognition and in situ amplification, capable of single-cell imaging mRNA at single-nucleotide and single-molecule resolution. Attributed to the use of Splint R ligase that can ligate padlock probe with RNA as target template, this method can efficiently detect mRNA in the absence of reverse transcription. This method enables spatial localization and correlation analysis of gene expression in single cells, which helps us to elucidate gene function and regulatory mechanisms.
摘要：
我们提出了一种基于基于连接的识别和原位扩增的直接mRNA检测的强大方法，能够以单核苷酸和单分子分辨率对mRNA进行单细胞成像。归因于使用可以将挂锁探针与RNA作为靶模板连接的夹板R连接酶，这种方法可以在没有逆转录的情况下有效地检测mRNA。该方法能够对单细胞中的基因表达进行空间定位和相关性分析，这有助于我们阐明基因功能和调控机制。
