Abstract：
Breast cancer, a prevalent disease with significant mortality rates, often presents treatment challenges due to its complex genetic makeup. This review explores the potential of combining Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) gene knockout strategies with immunotherapeutic approaches to enhance breast cancer treatment. The CRISPR/Cas9 system, renowned for its precision in inducing genetic alterations, can target and eliminate specific cancer cells, thereby minimizing off-target effects. Concurrently, immunotherapy, which leverages the immune system\'s power to combat cancer, has shown promise in treating breast cancer. By integrating these two strategies, we can potentially augment the effectiveness of immunotherapies by knocking out genes that enable cancer cells to evade the immune system. However, safety considerations, such as off-target effects and immune responses, necessitate careful evaluation. Current research endeavors aim to optimize these strategies and ascertain the most effective methods to stimulate the immune response. This review provides novel insights into the integration of CRISPR/Cas9-mediated knockout strategies and immunotherapy, a promising avenue that could revolutionize breast cancer treatment as our understanding of the immune system\'s interplay with cancer deepens.
摘要：
乳腺癌,一种死亡率很高的流行疾病，由于其复杂的遗传组成，经常提出治疗挑战。这篇综述探讨了将成簇的定期间隔短回文重复（CRISPR）/CRISPR相关蛋白9（Cas9）基因敲除策略与免疫治疗方法相结合以增强乳腺癌治疗的潜力。CRISPR/Cas9系统,以其诱导遗传改变的精确性而闻名，可以靶向并消除特定的癌细胞，从而最小化脱靶效应。同时,免疫疗法，它利用免疫系统的力量来对抗癌症，在治疗乳腺癌方面显示出了希望。通过整合这两种策略，我们可以通过敲除使癌细胞逃避免疫系统的基因来增强免疫疗法的有效性。然而,安全考虑,如脱靶效应和免疫反应，需要仔细评估。当前的研究努力旨在优化这些策略并确定刺激免疫反应的最有效方法。这篇综述为CRISPR/Cas9介导的基因敲除策略和免疫治疗的整合提供了新的见解。随着我们对免疫系统与癌症相互作用的理解加深，这条有希望的途径可能彻底改变乳腺癌治疗。
