Abstract：
A Gram-stain-negative, facultative anaerobe, rod-shaped strain JX-1T was isolated from UASB sludge treating landfill leachate in Wuhan, China. The isolate is capable of growing under conditions of pH 6.0-11.0 (optimum, pH 7.0-8.0), temperature 4-42 ℃ (optimum, 20-30 ℃), 0-8.0% (w/v) NaCl (optimum, 5.0%), and ammonia nitrogen concentration of 200-5000 mg/L (optimum, 500 mg/L) on LB plates. The microorganism can utilize malic acid, D-galactose, L-rhamnose, inosine, and L-glutamic acid as carbon sources, but does not reduce nitrates and nitrites. The major fatty acids are C18:1ω7c/C18:1ω6c, iso-C15:0, and anteiso-C15:0. The respiratory quinones are Q9 (91.92%) and Q8 (8.08%). Polar lipids include aminolipid, aminophospholipid, diphosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylglycerol, and phospholipid. Compared with other strains, strain JX-1T and Denitrificimonas caeni HY-14T have the highest values in terms of 16S rRNA gene sequence similarity (96.79%), average nucleotide identity (ANI; 76.06%), and average amino acid identity (AAI; 78.89%). Its digital DNA-DNA hybridization (dDDH) result is 20.3%. The genome of strain JX-1T, with a size of 2.78 Mb and 46.12 mol% G + C content, lacks genes for denitrification and dissimilatory nitrate reduction to ammonium (DNRA), but contains genes for ectoine synthesis as a secondary metabolite. The results of this polyphasic study allow genotypic and phenotypic differentiation of the analysed strain from the closest related species and confirm that the strain represents a novel species within the genus Denitrificimonas, for which the name Denitrificimonas halotolerans sp. nov. is proposed with JX-1T (= MCCC 1K08958T = KCTC 8395T) as the type strain.
摘要：
革兰氏染色阴性，兼性厌氧菌，从武汉UASB处理垃圾渗滤液的污泥中分离出棒状菌株JX-1T，中国。该分离物能够在pH6.0-11.0的条件下生长（最佳，pH7.0-8.0)，温度4-42℃(最佳，20-30℃),0-8.0%(w/v)NaCl(最佳，5.0%），和氨氮浓度为200-5000mg/L(最佳，500mg/L）在LB平板上。微生物可以利用苹果酸，D-半乳糖,L-鼠李糖,肌苷,和L-谷氨酸作为碳源，但不减少硝酸盐和亚硝酸盐。主要脂肪酸为C18：1ω7c/C18：1ω6c，iso-C15:0和anteiso-C15:0。呼吸醌是Q9(91.92%)和Q8(8.08%)。极性脂质包括氨基脂质，氨基磷脂，二磷脂酰甘油，糖脂,磷脂酰乙醇胺，磷脂酰甘油，和磷脂。与其他菌株相比，菌株JX-1T和反硝化单胞菌HY-14T在16SrRNA基因序列相似性方面具有最高值(96.79%),平均核苷酸同一性(ANI；76.06%)，和平均氨基酸同一性(AAI;78.89%)。其数字DNA-DNA杂交(dDDH)结果为20.3%。JX-1T菌株的基因组,尺寸为2.78Mb，G+C含量为46.12mol%，缺乏反硝化和异化硝酸盐还原成铵（DNRA）的基因，但含有作为次生代谢产物的外胎素合成基因。这项多相研究的结果允许所分析的菌株与最密切相关的物种的基因型和表型分化，并证实该菌株代表了Denitrificimonas属中的新物种，名称为Denitrificimonashalotoleranssp.11月。以JX-1T（=MCCC1K08958T=KCTC8395T）为类型菌株。
