PDF(Pubmed)
Abstract：
BACKGROUND: Campylobacter (C.) jejuni is one of the most important bacterial foodborne pathogens worldwide. Probiotics such as Lactobacillus or Bacillus species are considered one option for reducing the colonization rate and magnitude in poultry, the most frequent source of human infections. Due to the lack of suitable avian in vitro models such as chicken intestinal cell lines, especially those derived from the cecum, most in vitro studies on C. jejuni host interaction have been conducted with human intestinal cell lines. In this study, we compared C. jejuni-cell interactions between primary chicken cecal cells and the human intestinal cell line Caco-2, which is derived from colorectal adenocarcinoma, and investigated possible interfering effects of selected probiotic candidates.
RESULTS: We detected differences in adhesion and invasion between the two tested gut cell types and between different C. jejuni strains. The probiotic inhibition of C. jejuni adhesion and invasion of human and avian gut cells was affected by host cell type, investigated C. jejuni strain and time points of probiotic treatment. Additionally, our results suggest a possible correlation between C. jejuni invasion and the detected increase in IL-6 mRNA expression.
CONCLUSIONS: Our results indicate distinct differences between avian and human gut cells in their interaction with C. jejuni. Therefore, data obtained in one host species on C. jejuni-host interaction may not easily be transferrable to another one. The factors influencing the variable efficacy of probiotic intervention in chicken and human derived cells should be investigated further.
RESULTS: We detected differences in adhesion and invasion between the two tested gut cell types and between different C. jejuni strains. The probiotic inhibition of C. jejuni adhesion and invasion of human and avian gut cells was affected by host cell type, investigated C. jejuni strain and time points of probiotic treatment. Additionally, our results suggest a possible correlation between C. jejuni invasion and the detected increase in IL-6 mRNA expression.
CONCLUSIONS: Our results indicate distinct differences between avian and human gut cells in their interaction with C. jejuni. Therefore, data obtained in one host species on C. jejuni-host interaction may not easily be transferrable to another one. The factors influencing the variable efficacy of probiotic intervention in chicken and human derived cells should be investigated further.
摘要：
背景：弯曲杆菌(C.)空肠是世界范围内最重要的细菌性食源性病原体之一。益生菌如乳杆菌或芽孢杆菌属物种被认为是降低家禽定植率和数量的一种选择。最常见的人类感染源。由于缺乏合适的禽类体外模型，如鸡肠细胞系，尤其是那些来源于盲肠的,大多数关于空肠弯曲杆菌宿主相互作用的体外研究已经与人肠细胞系进行。在这项研究中,我们比较了原代鸡盲肠细胞和人肠道细胞系Caco-2之间的空肠弯曲杆菌细胞相互作用，并调查了选定的益生菌候选物可能的干扰作用。
结果：我们检测到两种测试的肠道细胞类型之间以及不同的空肠弯曲杆菌菌株之间的粘附和侵袭差异。益生菌对空肠弯曲杆菌粘附和侵袭人和禽肠细胞的抑制作用受宿主细胞类型的影响，研究了空肠杆菌菌株和益生菌治疗的时间点。此外,我们的结果表明空肠弯曲杆菌侵袭与检测到的IL-6mRNA表达增加之间可能存在相关性.
结论：我们的结果表明禽类和人肠道细胞在与空肠弯曲杆菌相互作用方面存在明显差异。因此,在一个宿主物种中获得的关于空肠弯曲杆菌与宿主相互作用的数据可能不容易转移到另一个。应进一步研究影响益生菌干预鸡源细胞和人源细胞的可变功效的因素。
结果：我们检测到两种测试的肠道细胞类型之间以及不同的空肠弯曲杆菌菌株之间的粘附和侵袭差异。益生菌对空肠弯曲杆菌粘附和侵袭人和禽肠细胞的抑制作用受宿主细胞类型的影响，研究了空肠杆菌菌株和益生菌治疗的时间点。此外,我们的结果表明空肠弯曲杆菌侵袭与检测到的IL-6mRNA表达增加之间可能存在相关性.
结论：我们的结果表明禽类和人肠道细胞在与空肠弯曲杆菌相互作用方面存在明显差异。因此,在一个宿主物种中获得的关于空肠弯曲杆菌与宿主相互作用的数据可能不容易转移到另一个。应进一步研究影响益生菌干预鸡源细胞和人源细胞的可变功效的因素。
