PDF(Pubmed)
Abstract：
BACKGROUND: Dental pathogens play a crucial role in oral health issues, including tooth decay, gum disease, and oral infections, and recent research suggests a link between these pathogens and oral cancer initiation and progression. Innovative therapeutic approaches are needed due to antibiotic resistance concerns and treatment limitations.
METHODS: We synthesized and analyzed piperine-coated zinc oxide nanoparticles (ZnO-PIP NPs) using UV spectroscopy, SEM, XRD, FTIR, and EDAX. Antioxidant and antimicrobial effectiveness were evaluated through DPPH, ABTS, and MIC assays, while the anticancer properties were assessed on KB oral squamous carcinoma cells.
RESULTS: ZnO-PIP NPs exhibited significant antioxidant activity and a MIC of 50 µg/mL against dental pathogens, indicating strong antimicrobial properties. Interaction analysis revealed high binding affinity with dental pathogens. ZnO-PIP NPs showed dose-dependent anticancer activity on KB cells, upregulating apoptotic genes BCL2, BAX, and P53.
CONCLUSIONS: This approach offers a multifaceted solution to combatting both oral infections and cancer, showcasing their potential for significant advancement in oral healthcare. It is essential to acknowledge potential limitations and challenges associated with the use of ZnO NPs in clinical applications. These may include concerns regarding nanoparticle toxicity, biocompatibility, and long-term safety. Further research and rigorous testing are warranted to address these issues and ensure the safe and effective translation of ZnO-PIP NPs into clinical practice.
METHODS: We synthesized and analyzed piperine-coated zinc oxide nanoparticles (ZnO-PIP NPs) using UV spectroscopy, SEM, XRD, FTIR, and EDAX. Antioxidant and antimicrobial effectiveness were evaluated through DPPH, ABTS, and MIC assays, while the anticancer properties were assessed on KB oral squamous carcinoma cells.
RESULTS: ZnO-PIP NPs exhibited significant antioxidant activity and a MIC of 50 µg/mL against dental pathogens, indicating strong antimicrobial properties. Interaction analysis revealed high binding affinity with dental pathogens. ZnO-PIP NPs showed dose-dependent anticancer activity on KB cells, upregulating apoptotic genes BCL2, BAX, and P53.
CONCLUSIONS: This approach offers a multifaceted solution to combatting both oral infections and cancer, showcasing their potential for significant advancement in oral healthcare. It is essential to acknowledge potential limitations and challenges associated with the use of ZnO NPs in clinical applications. These may include concerns regarding nanoparticle toxicity, biocompatibility, and long-term safety. Further research and rigorous testing are warranted to address these issues and ensure the safe and effective translation of ZnO-PIP NPs into clinical practice.
摘要：
背景：口腔病原体在口腔健康问题中起着至关重要的作用，包括蛀牙,牙龈疾病,和口腔感染，最近的研究表明，这些病原体与口腔癌的发生和进展之间存在联系。由于抗生素耐药性问题和治疗限制，需要创新的治疗方法。
方法：我们使用紫外光谱法合成并分析了胡椒碱包覆的氧化锌纳米颗粒（ZnO-PIPNP），SEM,XRD,FTIR,EDAX通过DPPH评估抗氧化和抗菌效果，ABTS,和MIC测定，而对KB口腔鳞状细胞癌细胞的抗癌特性进行了评估。
结果：ZnO-PIPNP表现出显着的抗氧化活性，对牙科病原体的MIC为50µg/mL，表明很强的抗菌性能。相互作用分析显示与牙科病原体的高结合亲和力。ZnO-PIPNP对KB细胞显示出剂量依赖性抗癌活性，上调凋亡基因BCL2、BAX、P53
结论：这种方法为对抗口腔感染和癌症提供了多方面的解决方案。展示他们在口腔保健方面取得重大进展的潜力。必须承认与在临床应用中使用ZnONP相关的潜在限制和挑战。这些可能包括有关纳米颗粒毒性的担忧，生物相容性，和长期安全。需要进一步的研究和严格的测试来解决这些问题，并确保将ZnO-PIPNP安全有效地转化为临床实践。
方法：我们使用紫外光谱法合成并分析了胡椒碱包覆的氧化锌纳米颗粒（ZnO-PIPNP），SEM,XRD,FTIR,EDAX通过DPPH评估抗氧化和抗菌效果，ABTS,和MIC测定，而对KB口腔鳞状细胞癌细胞的抗癌特性进行了评估。
结果：ZnO-PIPNP表现出显着的抗氧化活性，对牙科病原体的MIC为50µg/mL，表明很强的抗菌性能。相互作用分析显示与牙科病原体的高结合亲和力。ZnO-PIPNP对KB细胞显示出剂量依赖性抗癌活性，上调凋亡基因BCL2、BAX、P53
结论：这种方法为对抗口腔感染和癌症提供了多方面的解决方案。展示他们在口腔保健方面取得重大进展的潜力。必须承认与在临床应用中使用ZnONP相关的潜在限制和挑战。这些可能包括有关纳米颗粒毒性的担忧，生物相容性，和长期安全。需要进一步的研究和严格的测试来解决这些问题，并确保将ZnO-PIPNP安全有效地转化为临床实践。
