PDF(Pubmed)
Abstract：
To acquire the ability to fertilize the egg, mammalian spermatozoa must undergo a series of changes occurring within the highly synchronized and specialized environment of the female reproductive tract, collectively known as capacitation. In an attempt to replicate this process in vitro, various culture media for mouse sperm were formulated over the past decades, sharing a similar overall composition but differing mainly in ion concentrations and metabolic substrates. The widespread use of the different media to study the mechanisms of capacitation might hinder a comprehensive understanding of this process, as the medium could become a confounding variable in the analysis. In this context, the present side-by-side study compares the influence of four commonly used culture media (FD, HTF and two TYH versions) on mouse sperm capacitation. We evaluated the induction of protein kinase A phosphorylation pathway, motility, hyperactivation and acrosome reaction. Additionally, in vitro fertilization and embryo development were also assessed. By analyzing these outcomes in two mouse colonies with different reproductive performance, our study provides critical insights to improve the global understanding of sperm function. The results obtained highlight the importance of considering variations in medium composition, and their potential implications for the future interpretation of results.
摘要：
为了获得使卵子受精的能力，哺乳动物精子必须在女性生殖道高度同步和专门的环境中发生一系列变化，统称为获能。为了在体外复制这个过程，在过去的几十年中，配制了各种用于小鼠精子的培养基，共享相似的总体组成，但主要在离子浓度和代谢底物方面有所不同。广泛使用不同的媒体来研究获能机制可能会阻碍对这一过程的全面理解，因为介质可能成为分析中的混杂变量。在这种情况下，本并排研究比较了四种常用培养基(FD，HTF和两个TYH版本）对小鼠精子获能的影响。我们评估了蛋白激酶A磷酸化途径的诱导，运动性,过度激活和顶体反应。此外,还评估了体外受精和胚胎发育。通过分析两个具有不同繁殖性能的小鼠菌落中的这些结果，我们的研究为提高全球对精子功能的认识提供了重要的见解.获得的结果突出了考虑培养基成分变化的重要性，以及它们对未来结果解释的潜在影响。
