Abstract:
Euphorbia, one of the largest genera of flowering plants, is well-known for containing many biofuel crops. Euphorbia tirucalli, an evergreen succulent mainly native to the Africa continent but cultivated worldwide, is a promising petroleum plant with high tolerance to drought and salt stress. However, the exploration of such an important plant resource is severely hampered by the lack of a reference genome. Here, we present the chromosome-level genome assembly of E. tirucalli using PacBio HiFi sequencing and Hi-C technology. Its genome size was approximately 745.62 Mb, with a contig N50 of 74.16 Mb. A total of 743.63 Mb (99.73%) of the assembled sequences were anchored to 10 chromosomes with a complete BUSCO score of 97.80%. Genome annotation revealed 26,304 protein-coding genes, and 76.37% of the genome was identified as repeat elements. The high-quality genome provides valuable genetic resources that would be useful for unraveling the genetic mechanisms of biofuel synthesis and evolutionary adaptation of E. tirucalli.
摘要:
大猩猩,开花植物最大的属之一,以含有许多生物燃料作物而闻名。大黄提鲁卡利,一种常绿多肉植物,主要原产于非洲大陆,但在世界各地种植,是一种很有前途的石油植物,对干旱和盐胁迫具有很高的耐受性。然而,缺乏参考基因组严重阻碍了对如此重要的植物资源的探索。这里,我们使用PacBioHiFi测序和Hi-C技术介绍了T细胞染色体水平的基因组组装。它的基因组大小约为745.62Mb,重叠群N50为74.16Mb。总共743.63Mb(99.73%)的组装序列锚定到10条染色体上,完整的BUSCO评分为97.80%。基因组注释揭示了26,304个蛋白质编码基因,76.37%的基因组被鉴定为重复元件。高质量的基因组提供了宝贵的遗传资源,可用于揭示生物燃料合成的遗传机制和E.tirucalli的进化适应。