PDF(Pubmed)
Abstract：
Acrossocheilus fasciatus is a stream-dwelling fish species of the Barbinae subfamily. It is valued for its colorfully striped appearance and delicious meat. This species is also characterized by apparent sexual dimorphism and toxic ovum. Biology and aquaculture researches of A. fasciatus are hindered by the lack of a high-quality reference genome. Here, we report chromosome-level genome assemblies of the male and female A. fasciatus. The HiFi-only genome assemblies for both female and male individuals were 899.13 Mb (N50 length of 32.58 Mb) and 885.68 Mb (N50 length of 33.06 Mb), respectively. Notably, a substantial proportion of the assembled sequences, accounting for 96.15% and 98.35% for female and male genomes, respectively, were successfully anchored onto 25 chromosomes utilizing Hi-C data. We annotated the female assembly as a reference genome and identified a total of 400.62 Mb (44.56%) repetitive sequences, 27,392 protein-coding genes, and 35,869 ncRNAs. The high-quality male and female reference genomes will provide genomic resources for developing sex-specific molecular markers, inform single-sex breeding, and elucidate genetic mechanisms of sexual dimorphism.
摘要：
Acrossocheilusfasciatus是Barbinae亚科的一种溪流鱼类。它因其色彩鲜艳的条纹外观和美味的肉类而受到重视。该物种的特征还在于明显的性二态和有毒的卵子。由于缺乏高质量的参考基因组，阻碍了筋膜A的生物学和水产养殖研究。这里,我们报道了男性和女性筋膜A的染色体水平基因组组装。女性和男性个体的仅HiFi基因组装配体为899.13Mb（N50长度为32.58Mb）和885.68Mb（N50长度为33.06Mb），分别。值得注意的是,相当大比例的组装序列，占女性和男性基因组的96.15%和98.35%，分别,利用Hi-C数据成功锚定到25条染色体上。我们将雌性装配注释为参考基因组，并鉴定了总共400.62Mb（44.56％）的重复序列，27,392个蛋白质编码基因，和35,869个ncRNAs。高质量的男性和女性参考基因组将为开发性别特异性分子标记提供基因组资源,告知单性别育种，并阐明性二态性的遗传机制。
