Abstract：
Hydrogen sulfide (H2S) is a pungent gas that is one of the key mediators of signal transduction in biological systems, and its presence is related to the freshness of some protein foods. Using phenothiazine derivatives as fluorophores and 2, 4-dinitrobenzene sulfonate (DNBS) fragments as reaction groups, a near-infrared (NIR) probe WX-HS for H2S identification was designed. With the addition of H2S, WX-HS appeared a strong fluorescence signal at 660 nm with short reaction time (90 s) and high sensitivity, and fluorescence state change from non-fluorescent to orange-red. In addition, WX-HS could effectively detect H2S produced during food oxidation. Based on its low cytotoxicity, the WX-HS probe further enabled the detection and imaging of H2S in A549 cells.
摘要：
硫化氢(H2S)是一种刺激性气体,是生物系统中信号转导的关键介质之一。它的存在与一些蛋白质食物的新鲜度有关。使用吩噻嗪衍生物作为荧光团，2，4-二硝基苯磺酸盐（DNBS）片段作为反应基团，设计了一种用于H2S识别的近红外（NIR）探头WX-HS。随着H2S的加入，WX-HS在660nm处出现强荧光信号,反应时间短(90s),灵敏度高,荧光状态从非荧光变为橙红色。此外,WX-HS能有效检测食品氧化过程中产生的H2S。基于其低细胞毒性，WX-HS探针进一步实现了A549细胞中H2S的检测和成像。
