Abstract：
A novel laccase mimic enzyme Cu-Mn with excellent photothermal properties was firstly prepared via a combination of hydrothermal and in situ synthesis. Cu-Mn nanozymes could catalyze the typical laccase substrate 2,4-dichlorophenol (2,4-DP) to generate the red quinone imine. Further, loading the MnO2 nanosheets with photothermal properties, Cu-Mn nanozymes possessed not only excellent laccase catalytic activity, but also high photothermal conversion efficiency. The presence of glutathione S-transferase (GST) recovered the glutathione (GSH)-induced weakness of the laccase activity and photothermal properties of Cu-Mn. Hence, a GST enzyme-regulated dual-mode sensing strategy was established based on Cu-Mn nanozymes. The detection limits of GST monitoring based on colorimetric and photothermal methods were 0.092 and 0.087 U/L with response times of 20 min and 8 min, respectively. Furthermore, the proposed method enabled the measuring of GST levels in human serum and was successfully employed in the primary evaluation of hepatitis patients. Another attraction, the impressive photothermal behavior also endowed the Cu-Mn nanozymes with promising antimicrobial properties, which exhibited significant antimicrobial effects against Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus). Unsurprisingly, multifunctional Cu-Mn nanozymes certainly explore new paths in biochemical analysis and antimicrobial applications.
摘要：
首先通过水热和原位合成相结合的方法制备了具有优异光热性能的新型漆酶模拟酶Cu-Mn。Cu-Mn纳米酶可以催化典型的漆酶底物2,4-二氯苯酚（2,4-DP）生成红色醌亚胺。Further,加载具有光热性质的MnO2纳米片，Cu-Mn纳米酶不仅具有优异的漆酶催化活性,而且光热转换效率高。谷胱甘肽S-转移酶（GST）的存在恢复了谷胱甘肽（GSH）诱导的漆酶活性和Cu-Mn光热特性的减弱。因此，建立了基于Cu-Mn纳米酶的GST酶调节双模式传感策略。基于比色和光热方法的GST监测的检出限为0.092和0.087U/L，响应时间为20分钟和8分钟，分别。此外，所提出的方法能够测量人血清中的GST水平,并成功用于肝炎患者的初步评估.另一个吸引力，令人印象深刻的光热行为也赋予了Cu-Mn纳米酶有前途的抗菌性能，对大肠杆菌(E.大肠杆菌)和金黄色葡萄球菌(S.金黄色葡萄球菌）。毫不奇怪,多功能Cu-Mn纳米酶无疑在生化分析和抗菌应用中探索了新的途径。
