Abstract：
Human intestinal microbiota plays a crucial role in converting secoisolariciresinol diglucoside, a lignan found in flaxseed, to enterodiol, which has a range of health benefits: antioxidative, antitumor, and estrogenic/anti-estrogenic effects. Given the high secoisolariciresinol diglucoside content in flaxseed cake, this study investigated the potential of co-fermenting flaxseed cake with fermented soybean product to isolate bacterial strains that effectively convert secoisolariciresinol diglucoside to enterodiol in a controlled environment (in vitro). The co-fermentation process with stinky tofu microbiota significantly altered the lignan, generating 12 intermediate lignan metabolites as identified by targeted metabolomics. One particular promising strain, ZB26, demonstrated an impressive ability to convert secoisolariciresinol diglucoside. It achieved a conversion rate of 87.42 ± 0.33%, with secoisolariciresinol and enterodiol generation rates of 94.22 ± 0.51% and 2.91 ± 0.03%, respectively. Further optimization revealed, under specific conditions (0.5 mM secoisolariciresinol diglucoside, pH 8, 30 °C for 3 days), ZB26 could convert an even higher percentage (97.75 ± 0.05%) of the secoisolariciresinol diglucoside to generate secoisolariciresinol (103.02 ± 0.16%) and enterodiol (3.18 ± 0.31%). These findings suggest that the identified strains ZB26 have promising potential for developing functional foods and ingredients enriched with lignans.
摘要：
人类肠道微生物群在转化secoisolariciresinol二葡萄糖苷中起着至关重要的作用，在亚麻籽中发现的木酚素，对于肠二醇，具有一系列健康益处：抗氧化，抗肿瘤，和雌激素/抗雌激素作用。考虑到亚麻籽饼中的高含量，这项研究调查了将亚麻籽饼与发酵大豆产品共发酵的潜力，以分离出在受控环境（体外）中有效地将secoisolariciresinol二葡萄糖苷转化为肠二醇的细菌菌株。与臭豆腐微生物群的共发酵过程显着改变了木酚素，产生12个通过靶向代谢组学鉴定的中间木酚素代谢物。一种特别有前途的菌株，ZB26表现出令人印象深刻的转化secoisolariciresinol二葡萄糖苷的能力。实现了87.42±0.33%的转化率,与己基异瑞林和肠二醇的产生率为94.22±0.51%和2.91±0.03%，分别。进一步优化显示，在特定条件下(0.5mM谢奥异赖辛醇二葡糖苷，pH8，30°C3天），ZB26可以转化甚至更高百分比（97.75±0.05％）的塞科isolaricisuresinol二葡糖苷以产生塞科isolaricisenesinol（103.02±0.16％）和肠二醇（3.18±0.31％）。这些发现表明，已鉴定的菌株ZB26具有开发富含木脂素的功能性食品和成分的潜力。
