Abstract：
Sulforaphane (SFaN) is a food-derived compound with several bioactive properties, including atherosclerosis, diabetes, and obesity treatment. However, the mechanisms by which SFaN exerts its various effects are still unclear. To elucidate the mechanisms of the various effects of SFaN, we explored novel SFaN-binding proteins using SFaN beads and identified acyl protein thioesterase 2 (APT2). We also found that SFaN binds to the APT2 via C56 residue and attenuates the palmitoylation of APT2, thereby reducing plasma membrane localization of APT2. This study reveals a novel bioactivity of SFaN as a regulator of APT2 protein palmitoylation.
摘要：
萝卜硫烷（SFaN）是一种具有多种生物活性的食品衍生化合物，包括动脉粥样硬化,糖尿病,和肥胖治疗。然而,SFaN发挥各种作用的机制尚不清楚。为了阐明SFN的各种影响的机制，我们使用SFN珠子探索了新的SFN结合蛋白，并鉴定了酰基蛋白硫酯酶2（APT2）。我们还发现，SFN通过C56残基与APT2结合，并减弱APT2的棕榈酰化，从而减少APT2的质膜定位。这项研究揭示了SFaN作为APT2蛋白棕榈酰化调节剂的新型生物活性。
