Abstract：
Herein we report the assessment of the effects of shockwave (SW) impacts on adult rat hippocampal progenitor cell (AHPC) neurospheres (NSs), which are used as in vitro brain models, for enhancing our understanding of the mechanisms of traumatic brain injury (TBI). The assessment has been achieved by using culture dishes and a new microchip. The microchip allows the chemicals released from the brain models cultured inside the cell culture chamber under SW impacts to diffuse to the nanosensors in adjacent sensor chambers through built-in diffusion barriers, which are used to prevent the cells from entering the sensor chambers, thereby mitigating the biofouling issues of the sensor surface. Experiments showed the negative impact of the SW on the viability, proliferation, and differentiation of the cells within the NSs. A qPCR gene expression analysis was performed and appeared to confirm some of the immunocytochemistry (ICC) results. Finally, we demonstrated that the microchip can be used to monitor lactate dehydrogenase (LDH) released from the AHPC-NSs subjected to SW impacts. As expected, LDH levels changed when AHPC-NSs were injured by SW impacts, verifying this chip can be used for assessing the degrees of injuries to AHPC-NSs by monitoring LDH levels. Taken together, these results suggest the feasibility of using the chip to better understand the interactions between SW impacts and in vitro brain models, paving the way for potentially establishing in vitro TBI models on a chip.
摘要：
在这里，我们报告了冲击波（SW）对成年大鼠海马祖细胞（AHPC）神经球（NSs）的影响的评估，它们被用作体外大脑模型，增强我们对创伤性脑损伤（TBI）机制的理解。通过使用培养皿和新的微芯片实现了评估。微芯片允许在SW冲击下从细胞培养室内培养的大脑模型释放的化学物质通过内置的扩散屏障扩散到相邻传感器室中的纳米传感器，用于防止细胞进入传感器室，从而减轻传感器表面的生物污染问题。实验表明，SW对生存能力的负面影响，扩散,和NS内细胞的分化。进行qPCR基因表达分析,似乎证实了一些免疫细胞化学(ICC)结果。最后,我们证明，微芯片可用于监测受SW影响的AHPC-NS释放的乳酸脱氢酶（LDH）。不出所料,当AHPC-NS受到SW撞击时，LDH水平发生变化，验证该芯片可用于通过监测LDH水平评估AHPC-NS的损伤程度。一起来看,这些结果表明，使用该芯片更好地了解SW影响和体外脑模型之间的相互作用的可行性，为在芯片上建立体外TBI模型铺平了道路。
