PDF(Pubmed)
Abstract：
UNASSIGNED: Using a pig model of cardiopulmonary bypass, we compared outcomes after cardioplegia either with our in-house \"Huaxi-1\" solution containing natural blood and crystalloid or with the entirely crystalloid, commercially available \"histidine-tryptophan-ketoglutarate\" solution.
UNASSIGNED: Cardiopulmonary bypass was established in 12 healthy male pigs, who were randomized to receive a single dose of either Huaxi-1 or entirely crystalloid. All animals were then subjected to whole-heart ischemia for 90 min, followed by 2 h of reperfusion, after which myocardial injury was assessed in terms of cardiac function, myocardial pathology and levels of biomarkers in plasma, while levels of high-energy phosphate in myocardium were assayed using liquid chromatography.
UNASSIGNED: Animals given Huaxi-1 cardioplegia required significantly less time to be weaned off bypass, they received significantly lower doses of norepinephrine, and they showed significantly higher levels (mean ± SD) of adenosine triphosphate (14 ± 4 vs. 8 ± 2 µg/mg, P = 0.005), adenosine diphosphate (16 ± 2 vs. 13 ± 2 µg/mg, P = 0.046), and total adenine nucleotide (37 ± 4 vs. 30 ± 3 µg/mg, P = 0.006) in myocardium after 2 h of reperfusion. They also showed less severe bleeding, edema and injury to mitochondria and myofibers in myocardium. The two groups did not differ significantly in doses of inotropic drugs received, cardiac output or levels of biomarkers in plasma.
UNASSIGNED: In this animal model of healthy hearts subjected to 90 min of ischemia, Huaxi-1 cardioplegia may be superior to entirely crystalloid cardioplegia for promoting energy generation and attenuating ischemia/reperfusion injury in myocardium.
UNASSIGNED: Cardiopulmonary bypass was established in 12 healthy male pigs, who were randomized to receive a single dose of either Huaxi-1 or entirely crystalloid. All animals were then subjected to whole-heart ischemia for 90 min, followed by 2 h of reperfusion, after which myocardial injury was assessed in terms of cardiac function, myocardial pathology and levels of biomarkers in plasma, while levels of high-energy phosphate in myocardium were assayed using liquid chromatography.
UNASSIGNED: Animals given Huaxi-1 cardioplegia required significantly less time to be weaned off bypass, they received significantly lower doses of norepinephrine, and they showed significantly higher levels (mean ± SD) of adenosine triphosphate (14 ± 4 vs. 8 ± 2 µg/mg, P = 0.005), adenosine diphosphate (16 ± 2 vs. 13 ± 2 µg/mg, P = 0.046), and total adenine nucleotide (37 ± 4 vs. 30 ± 3 µg/mg, P = 0.006) in myocardium after 2 h of reperfusion. They also showed less severe bleeding, edema and injury to mitochondria and myofibers in myocardium. The two groups did not differ significantly in doses of inotropic drugs received, cardiac output or levels of biomarkers in plasma.
UNASSIGNED: In this animal model of healthy hearts subjected to 90 min of ischemia, Huaxi-1 cardioplegia may be superior to entirely crystalloid cardioplegia for promoting energy generation and attenuating ischemia/reperfusion injury in myocardium.
摘要：
■使用猪体外循环模型，我们比较心脏停搏后的结果与我们的内部\"华西-1\"溶液含有天然血液和晶体或与完全晶体，市售“组氨酸-色氨酸-酮戊二酸”溶液。
■在12只健康的雄性猪中建立了体外循环，他们被随机分配接受单剂量的华西-1或完全晶体。然后将所有动物进行全心脏缺血90分钟，然后再灌注2小时，然后根据心脏功能评估心肌损伤，心肌病理学和血浆中生物标志物的水平，同时使用液相色谱法测定心肌中的高能磷酸盐水平。
■给予华西-1心脏停搏液的动物需要的断奶时间明显减少，他们接受了明显较低剂量的去甲肾上腺素,并且它们显示出三磷酸腺苷的水平（平均值±SD）（14±4与8±2微克/毫克，P=0.005),二磷酸腺苷(16±2vs.13±2微克/毫克，P=0.046),和总腺嘌呤核苷酸(37±4vs.30±3微克/毫克，再灌注2h后心肌P=0.006）。他们还显示出血不太严重，心肌线粒体和肌纤维的水肿和损伤。两组接受的正性肌力药物的剂量没有显着差异，心输出量或血浆生物标志物水平。
■在这种健康心脏缺血90分钟的动物模型中，在促进心肌能量生成和减轻缺血/再灌注损伤方面，华西-1心脏停搏液可能优于完全晶体心脏停搏液。
■在12只健康的雄性猪中建立了体外循环，他们被随机分配接受单剂量的华西-1或完全晶体。然后将所有动物进行全心脏缺血90分钟，然后再灌注2小时，然后根据心脏功能评估心肌损伤，心肌病理学和血浆中生物标志物的水平，同时使用液相色谱法测定心肌中的高能磷酸盐水平。
■给予华西-1心脏停搏液的动物需要的断奶时间明显减少，他们接受了明显较低剂量的去甲肾上腺素,并且它们显示出三磷酸腺苷的水平（平均值±SD）（14±4与8±2微克/毫克，P=0.005),二磷酸腺苷(16±2vs.13±2微克/毫克，P=0.046),和总腺嘌呤核苷酸(37±4vs.30±3微克/毫克，再灌注2h后心肌P=0.006）。他们还显示出血不太严重，心肌线粒体和肌纤维的水肿和损伤。两组接受的正性肌力药物的剂量没有显着差异，心输出量或血浆生物标志物水平。
■在这种健康心脏缺血90分钟的动物模型中，在促进心肌能量生成和减轻缺血/再灌注损伤方面，华西-1心脏停搏液可能优于完全晶体心脏停搏液。
