UNASSIGNED: We induced COPD in C57BL/6 mice through an orotracheal application of cigarette smoke extract. PMB treatment was applied for the entire 7 weeks and Bronchoalveolar lavage (BAL) and lungs were collected to study production of IFN- γ and IL-10 in the lung. After the last administration with cigarette smoke extract (end of 7 weeks), 24 h later, the animals were euthanized. One-way ANOVA followed by NewmanKeuls test were used for statistical analysis with significance levels adjusted to 5% (p < 0.05).
UNASSIGNED: This result showed that PBM improves COPD symptomatology, reducing the number of inflammatory cells (macrophages, neutrophils and lymphocytes), the levels of IFN-γ among others, and increased IL-10. We also observed a decrease of collagen, mucus, bronchoconstriction index, alveolar enlargement, CD4+, CD8+, CD4+STAT4+, and CD4+IFN-γ+ cells. In addition, in the treated group, we found an increase in CD4+CD25+Foxp3+ and CD4+IL-10+ T cells.
UNASSIGNED: This study suggests that PBM treatment could be applied as an immunotherapeutic strategy for COPD.
■我们通过气管应用香烟烟雾提取物在C57BL/6小鼠中诱导COPD。应用PMB治疗整个7周,收集支气管肺泡灌洗(BAL)和肺以研究肺中IFN-γ和IL-10的产生。最后一次服用香烟烟雾提取物后(7周结束),24小时后,动物被安乐死。单因素方差分析和NewmanKeuls检验用于统计学分析,显著性水平调整至5%(p<0.05)。
■该结果表明PBM改善了COPD症状,减少炎症细胞的数量(巨噬细胞,中性粒细胞和淋巴细胞),IFN-γ的水平,IL-10增加。我们还观察到胶原蛋白的减少,粘液,支气管收缩指数,肺泡肿大,CD4+,CD8+,CD4+STAT4+,和CD4+IFN-γ+细胞。此外,在治疗组中,我们发现CD4+CD25+Foxp3+和CD4+IL-10+T细胞增加。
■本研究提示PBM治疗可作为COPD的免疫治疗策略。