PDF(Pubmed)
Abstract：
UNASSIGNED: Nucleotide-binding domain (NOD)-like receptors (NLRs) are expressed in the endometrium, and involved in modulating the female innate immune responses. There are conceptus-endometrial interactions during pregnancy, which ensure immune homeostasis of the maternal-fetal interface. The purpose of this study was to explore the effects of early pregnancy on NLR expression in the ovine endometrium.
UNASSIGNED: Endometrial tissues were collected at day 16 of the estrous cycle, and at days 13, 16 and 25 of pregnancy (n = 6 for each group), and RT-qPCR, western blot and immunohistochemistry analysis were used to analyze the expression of NLRs, including NOD1, NOD2, major histocompatibility complex class II transactivator (CIITA), neuronal apoptosis inhibitor protein (NAIP), NLR family, pyrin domain-containing 1 (NLRP1), NLRP3 and NLRP7.
UNASSIGNED: Expression levels of NOD1, NOD2, NAIP, CIITA, NLRP1 and NLRP3 declined, but expression level of NLRP7 increased in the endometria during early pregnancy compared with nonpregnant ewes. In addition, NOD2 and CIITA proteins were located in the endometrium in a protein type-, cell type- and pregnancy status-specific manner.
UNASSIGNED: Early pregnancy modulated expression of NLR family in the ovine endometrium, which may be essential for conceptus-endometrial interactions and maternal-fetal interface immune homeostasis.
UNASSIGNED: Endometrial tissues were collected at day 16 of the estrous cycle, and at days 13, 16 and 25 of pregnancy (n = 6 for each group), and RT-qPCR, western blot and immunohistochemistry analysis were used to analyze the expression of NLRs, including NOD1, NOD2, major histocompatibility complex class II transactivator (CIITA), neuronal apoptosis inhibitor protein (NAIP), NLR family, pyrin domain-containing 1 (NLRP1), NLRP3 and NLRP7.
UNASSIGNED: Expression levels of NOD1, NOD2, NAIP, CIITA, NLRP1 and NLRP3 declined, but expression level of NLRP7 increased in the endometria during early pregnancy compared with nonpregnant ewes. In addition, NOD2 and CIITA proteins were located in the endometrium in a protein type-, cell type- and pregnancy status-specific manner.
UNASSIGNED: Early pregnancy modulated expression of NLR family in the ovine endometrium, which may be essential for conceptus-endometrial interactions and maternal-fetal interface immune homeostasis.
摘要：
■核苷酸结合域（NOD）样受体（NLR）在子宫内膜中表达，并参与调节女性先天免疫反应。怀孕期间有概念-子宫内膜相互作用，确保母胎界面的免疫稳态。目的探讨早期妊娠对绵羊子宫内膜NLR表达的影响。
■在发情周期的第16天收集子宫内膜组织，在怀孕的第13、16和25天（每组n=6），和RT-qPCR，Westernblot和免疫组化分析NLRs的表达,包括NOD1，NOD2，主要组织相容性复合物II类反式激活因子（CIITA），神经元凋亡抑制蛋白（NAIP），NLR家族,含pyrin结构域1（NLRP1），NLRP3和NLRP7。
■NOD1、NOD2、NAIP、CIITA,NLRP1和NLRP3下降，但与未怀孕的母羊相比，妊娠早期子宫内膜中NLRP7的表达水平增加。此外,NOD2和CIITA蛋白位于子宫内膜的蛋白质类型-，细胞类型和妊娠状态特异性方式。
■早期妊娠调节NLR家族在绵羊子宫内膜中的表达，这对于概念-子宫内膜相互作用和母胎界面免疫稳态可能是必不可少的。
■在发情周期的第16天收集子宫内膜组织，在怀孕的第13、16和25天（每组n=6），和RT-qPCR，Westernblot和免疫组化分析NLRs的表达,包括NOD1，NOD2，主要组织相容性复合物II类反式激活因子（CIITA），神经元凋亡抑制蛋白（NAIP），NLR家族,含pyrin结构域1（NLRP1），NLRP3和NLRP7。
■NOD1、NOD2、NAIP、CIITA,NLRP1和NLRP3下降，但与未怀孕的母羊相比，妊娠早期子宫内膜中NLRP7的表达水平增加。此外,NOD2和CIITA蛋白位于子宫内膜的蛋白质类型-，细胞类型和妊娠状态特异性方式。
■早期妊娠调节NLR家族在绵羊子宫内膜中的表达，这对于概念-子宫内膜相互作用和母胎界面免疫稳态可能是必不可少的。
