PDF(Pubmed)
Abstract：
UNASSIGNED: Sarcocystis includes a global group of apicomplexan parasites with two-host life cycle frequently circulating in wildlife and domestic hosts, including humans. Two of the most important wild terrestrial carnivores acting as definitive hosts are the red fox and raccoon dog, due to their wide distribution in Europe and usage of wild and farmed animals as prey. This study was conducted to determine the prevalence of Sarcocystis in hunted red foxes and raccoon dogs from nine regions of the Czech Republic and to identify isolated sporocysts by molecular techniques.
UNASSIGNED: Approximately 5 g of the contents of large intestine from 200 animals (197 red foxes and three raccoon dogs) were examined by flotation centrifugation coprological method. Only samples of 50 red foxes and one raccoon dog positive to Sarcocystis spp. were used for the nested PCR (nPCR) method to amplify a fragment or partial sequence on the cox1 gene. Ten species-specific primer pairs for detection of Sarcocystis spp. using farm animals as intermediate hosts were utilized.
UNASSIGNED: In total, 38.1% of the red foxes and 66.7% of the raccoon dogs were positive to Sarcocystis by light microscopy. The molecular characterization resulted in the identification of five species in the red fox: S. arieticanis, S. capracanis, S. cruzi, S. miescheriana, and S. tenella, while the PCR was negative for the sole raccoon dog. The highest intraspecific variation was found for S. miescheriana, while S. tenella was the most prevalent. Co-infections occurred in the large intestine of the red fox. No zoonotic species were found in our samples.
UNASSIGNED: This is the first study where the potential role of the red fox and raccoon dogs as spreaders of Sarcocystis to farm animals in the Czech Republic is shown. The use of species-specific primers provides a fast and easy method for screening multiple samples for a particular Sarcocystis species.
UNASSIGNED: Approximately 5 g of the contents of large intestine from 200 animals (197 red foxes and three raccoon dogs) were examined by flotation centrifugation coprological method. Only samples of 50 red foxes and one raccoon dog positive to Sarcocystis spp. were used for the nested PCR (nPCR) method to amplify a fragment or partial sequence on the cox1 gene. Ten species-specific primer pairs for detection of Sarcocystis spp. using farm animals as intermediate hosts were utilized.
UNASSIGNED: In total, 38.1% of the red foxes and 66.7% of the raccoon dogs were positive to Sarcocystis by light microscopy. The molecular characterization resulted in the identification of five species in the red fox: S. arieticanis, S. capracanis, S. cruzi, S. miescheriana, and S. tenella, while the PCR was negative for the sole raccoon dog. The highest intraspecific variation was found for S. miescheriana, while S. tenella was the most prevalent. Co-infections occurred in the large intestine of the red fox. No zoonotic species were found in our samples.
UNASSIGNED: This is the first study where the potential role of the red fox and raccoon dogs as spreaders of Sarcocystis to farm animals in the Czech Republic is shown. The use of species-specific primers provides a fast and easy method for screening multiple samples for a particular Sarcocystis species.
摘要：
■Sarcocystis包括一组全球性的顶复星寄生虫，其两个宿主的生命周期经常在野生动物和家庭宿主中循环，包括人类。作为确定寄主的两种最重要的野生陆地食肉动物是红狐狸和浣熊狗，由于它们在欧洲的广泛分布以及使用野生和养殖动物作为猎物。进行这项研究是为了确定捷克共和国9个地区的猎杀赤狐和浣熊犬中的肉孢子虫的患病率，并通过分子技术鉴定分离的孢子囊。
■通过浮选离心法检查了200只动物（197只红狐狸和三只浣熊犬）的约5g大肠内容物。仅50只红狐狸和一只浣熊犬的样本对肉囊菌属呈阳性。用于巢式PCR（nPCR）方法，以扩增cox1基因上的片段或部分序列。十种特异性引物对，用于检测结节虫。利用农场动物作为中间宿主。
■总共，通过光学显微镜检查，38.1%的红狐狸和66.7%的浣熊犬对结节虫呈阳性。分子表征导致鉴定了红狐狸中的五个物种：S.arieticanis，美国卡普拉卡尼斯，S.Cruzi,S.Miescheriana,还有S.Tenella,而唯一的浣熊狗的PCR是阴性的。最高的种内变异是S.miescheriana，而美国是最普遍的。共感染发生在红狐狸的大肠中。在我们的样本中没有发现人畜共患物种。
■这是第一项研究，其中显示了红狐狸和浣熊狗作为肉孢子虫向捷克共和国农场动物传播的潜在作用。物种特异性引物的使用提供了一种快速简便的方法，用于筛选多个样品中特定的孢子虫物种。
■通过浮选离心法检查了200只动物（197只红狐狸和三只浣熊犬）的约5g大肠内容物。仅50只红狐狸和一只浣熊犬的样本对肉囊菌属呈阳性。用于巢式PCR（nPCR）方法，以扩增cox1基因上的片段或部分序列。十种特异性引物对，用于检测结节虫。利用农场动物作为中间宿主。
■总共，通过光学显微镜检查，38.1%的红狐狸和66.7%的浣熊犬对结节虫呈阳性。分子表征导致鉴定了红狐狸中的五个物种：S.arieticanis，美国卡普拉卡尼斯，S.Cruzi,S.Miescheriana,还有S.Tenella,而唯一的浣熊狗的PCR是阴性的。最高的种内变异是S.miescheriana，而美国是最普遍的。共感染发生在红狐狸的大肠中。在我们的样本中没有发现人畜共患物种。
■这是第一项研究，其中显示了红狐狸和浣熊狗作为肉孢子虫向捷克共和国农场动物传播的潜在作用。物种特异性引物的使用提供了一种快速简便的方法，用于筛选多个样品中特定的孢子虫物种。
