PDF(Pubmed)
Abstract：
Lately, the potential risk of disease transmission due to the use of bovine-derived bone substitutes has become obvious, demonstrating the urgent need for a synthetic grafting material with comparable bioactive behaviour and properties. Therefore, the effect of a synthetic hydroxyapatite (HA) (Osbone®) bone grafting material on bone regeneration was evaluated 2 weeks, 1 month, and 3, 6, 12 and 18 months after implantation in critical-size bone defects in the ovine scapula and compared to that of a bovine-derived HA (Bio-Oss®) and β-tricalcium phosphate (TCP) (Cerasorb® M). New bone formation and the biodegradability of the bone substitutes were assessed histomorphometrically. Hard tissue histology and immunohistochemical analysis were employed to characterize collagen type I, alkaline phosphatase, osteocalcin, as well as bone sialoprotein expression in the various cell and matrix components of the bone tissue to evaluate the bioactive properties of the bone grafting materials. No inflammatory tissue response was detected with any of the bone substitute materials studied. After 3 and 6 months, β-TCP (Cerasorb® M) showed superior bone formation when compared to both HA-based materials (3 months: β-TCP 55.65 ± 2.03% vs. SHA 49.05 ± 3.84% and BHA 47.59 ± 1.97%; p ≤ 0.03; 6 months: β-TCP 62.03 ± 1.58%; SHA: 55.83 ± 2.59%; BHA: 53.44 ± 0.78%; p ≤ 0.04). Further, after 12 and 18 months, a similar degree of bone formation and bone-particle contact was noted for all three bone substitute materials without any significant differences. The synthetic HA supported new bone formation, osteogenic marker expression, matrix mineralization and good bone-bonding behaviour to an equal and even slightly superior degree compared to the bovine-derived HA. As a result, synthetic HA can be regarded as a valuable alternative to the bovine-derived HA without the potential risk of disease transmission.
摘要：
最近,由于使用牛源性骨替代品而导致疾病传播的潜在风险已经变得显而易见，表明迫切需要具有可比的生物活性行为和性能的合成接枝材料。因此,2周评估了合成羟基磷灰石（HA）（Osbone®）骨移植材料对骨再生的影响，1个月,和植入后3、6、12和18个月，在绵羊肩胛骨的临界尺寸骨缺损中，并与牛衍生的HA（Bio-Oss®）和β-磷酸三钙（TCP）（Cerasorb®M）进行比较。通过组织形态来评估新骨形成和骨替代物的生物降解性。硬组织组织学和免疫组织化学分析用于表征I型胶原，碱性磷酸酶,骨钙蛋白，以及骨唾液酸蛋白在骨组织的各种细胞和基质成分中的表达，以评估骨移植材料的生物活性。研究的任何骨替代材料均未检测到炎症组织反应。3个月和6个月后，与两种基于HA的材料相比，β-TCP（Cerasorb®M）显示出较好的骨形成（3个月：β-TCP55.65±2.03％vs.SHA49.05±3.84％，BHA47.59±1.97％；p≤0.03；6个月：β-TCP62.03±1.58％；SHA：55.83±2.59％；BHA：53.44±0.78％；p≤0.04）。Further,12个月和18个月后，三种骨替代材料的骨形成和骨-颗粒接触程度相似,无显著差异.合成HA支持新骨形成，成骨标记表达，与牛衍生的HA相比，基质矿化和良好的骨结合行为达到相等甚至更优越的程度。因此,合成HA可以被认为是牛源HA的有价值的替代品，没有潜在的疾病传播风险。
