PDF(Pubmed)
Abstract：
Neurodegenerative diseases (NDs) are pathological conditions initiated by the loss of neuronal cell structure and the progressive decline in function caused by prolonged neuroinflammation. Postmenopausal women are at a high risk of experiencing NDs due to estrogen deficiency in their bodies, necessitating the administration of phytoestrogens as a replacement for estrogen in the body. One alternative therapy is administering phytoestrogens, estrogen-like substances from plants, which can be obtained from Marsilea crenata C. Presl. leaves. The purpose of this study was to determine whether administration of the n-butanol fraction (BF) and water fraction (WF) of M. crenata leaves could increase locomotor activity in rotenone-induced zebrafish. Treatment was given to each group of zebrafish with BF and WF at doses of 2.5; 5; 10; and 20 ppm to determine the locomotor activity. Then an analysis was carried out by looking at each movement of the zebrafish swimming for 1 min at the time of observation on days 0, 7, 14, 21, and 28. The result showed that BF and WF significantly increased the locomotor activity of zebrafish at the optimum dose of 20 ppm for BF and 5 ppm for WF compared to the negative control. This concludes that the polar fraction of M. crenata leaves is proven to have the potential to prevent ND progressivity.
摘要：
神经退行性疾病（ND）是由神经元细胞结构的丧失和由长期神经炎症引起的功能进行性下降引发的病理状况。绝经后妇女由于体内雌激素缺乏而有很高的风险，需要施用植物雌激素作为体内雌激素的替代品。一种替代疗法是给予植物雌激素，来自植物的雌激素样物质，其可以从MarsileacrenataC.Presl获得。叶子。这项研究的目的是确定在鱼藤酮诱导的斑马鱼中施用M.crenata叶的正丁醇部分（BF）和水部分（WF）是否可以增加运动活性。以2.5、5、10和20ppm的剂量对每组斑马鱼给予BF和WF治疗以确定运动活性。然后通过观察在第0、7、14、21和28天观察时斑马鱼游泳1分钟的每个动作进行分析。结果表明，与阴性对照相比，BF和WF在BF20ppm和WF5ppm的最佳剂量下显着增加斑马鱼的运动活性。这得出结论,M.crenata叶的极性部分被证明具有防止ND进展的潜力。
