PDF(Pubmed)
Abstract：
This research investigated the anticancer properties of punicalagin, a prominent bioactive polyphenol extracted from Punica granatum L, in human gastric cancer cell lines. Normal and gastric cancer cells were exposed to different doses of punicalagin for various durations. Punicalagin exhibited cytotoxic effects on gastric cancer cells in a dose- and time-dependent fashion, while sparing normal gastric epithelial cells. It is noteworthy that among the 3 gastric cancer cells, HGC-27 cells were more resistant to punicalagin than 23,132/87 and AGS cells. Furthermore, punicalagin triggered apoptosis in gastric cancer cells, evidenced by a rise in both early and late apoptotic cell percentages. Western blot analysis further revealed that punicalagin elevated the levels of activated caspase-3. Conversely, punicalagin curtailed cell invasion and reduced the expression of MMP-2, MMP-9, Snail, and Slug. From a mechanistic standpoint, Western blotting indicated that punicalagin might inhibit the Erk and NF-κB pathways, leading to apoptosis induction and the inhibition of cell invasion in gastric cancer cells. These results indicate that punicalagin promotes apoptosis and inhibits cell invasion in gastric cancer cells by activating caspase-3 and suppressing MMP-2, MMP-9, Snail, and Slug through the inhibition of the Erk and NF-κB pathways.
摘要：
这项研究调查了punicalagin的抗癌特性，一种从石榴中提取的突出的生物活性多酚，在人类胃癌细胞系中。将正常和胃癌细胞暴露于不同剂量的punicalagin持续不同的持续时间。Punicalagin以剂量和时间依赖性方式对胃癌细胞表现出细胞毒性作用，同时保留正常的胃上皮细胞。值得注意的是，在3个胃癌细胞中，HGC-27细胞对punicalagin的抗性高于23,132/87和AGS细胞。此外，punicalagin触发胃癌细胞凋亡，早期和晚期凋亡细胞百分比均上升。Western印迹分析进一步显示punicalagin升高了活化的caspase-3的水平。相反，punicalagin抑制细胞侵袭,降低MMP-2,MMP-9,蜗牛,还有Slug.从机械的角度来看，Western印迹表明punicalagin可能抑制Erk和NF-κB通路，导致胃癌细胞凋亡诱导和细胞侵袭抑制。这些结果表明punicalagin通过激活caspase-3并抑制MMP-2，MMP-9，Snail促进胃癌细胞凋亡并抑制细胞侵袭，和Slug通过抑制Erk和NF-κB途径。
