RESULTS: Here, we identified a total of 13 OfYABBY genes from the Osmanthus fragrans genome. These 13 OfYABBY genes were divided into five subfamilies through phylogenetic analysis, and genes in the same subfamily showed similar gene structures and conserved protein motifs. Gene duplication promoted the expansion of the OfYABBY family in O. fragrans. Tissue-specific expression analysis showed that the OfYABBY family was mainly expressed in O. fragrans leaves and floral organs. To better understand the role of OfYABBY genes in plant growth and development, OfYABBY12 was selected for heterologous stable overexpression in tobacco, and OfYABBY12-overexpressing tobacco leaves released significantly fewer volatile organic compounds than wild-type tobacco leaves. Overexpression of OfYABBY12 led to the downregulation of NtCCD1/4 and decreased β-ionone biosynthesis. Correspondingly, a dual-luciferase assay showed that OfYABBY12 negatively regulated the expression of OfCCD4, which promotes β-ionone synthesis. Furthermore, tobacco leaves overexpressing OfYABBY12 were curled and wrinkled and had significantly reduced leaf thickness and leaf inclusions and significantly extended flower pistils (styles).
CONCLUSIONS: Overall, the results suggest that the OfYABBY gene family may influence the biosynthesis of the floral scent (especially β-ionone) in O. fragrans and may regulate leaf morphogenesis and lateral organs.
结果:这里,我们从桂花基因组中鉴定出13个OfYABBY基因.这13个YABBY基因通过系统发育分析分为5个亚家族,并且同一亚家族中的基因显示出相似的基因结构和保守的蛋白质基序。基因复制促进了OfYABBY家族在O.fragrans中的扩展。组织特异性表达分析表明,OfYABBY家族主要在O.fragrans叶片和花器官中表达。为了更好地理解OfYABBY基因在植物生长发育中的作用,OfYABBY12被选择用于烟草中异源稳定的过表达,和OfYABBY12过表达的烟叶释放的挥发性有机化合物明显少于野生型烟叶。OfYABBY12的过表达导致NtCCD1/4的下调和β-紫罗兰酮生物合成的减少。相应地,双荧光素酶实验表明,OfYABBY12负调控OfCCD4的表达,促进β-紫罗兰酮的合成.此外,过表达YABBY12的烟草叶片卷曲和起皱,叶片厚度和叶片内含物显着降低,花雌蕊(花柱)显着延长。
结论:总体而言,结果表明,OfYABBY基因家族可能会影响O.花香中花香(尤其是β-紫罗兰酮)的生物合成,并可能调节叶片形态发生和侧生器官。