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Abstract：
Acute myeloid leukaemia (AML) is an aggressive leukaemia characterised by uncontrolled blast cell proliferation. miRNAs and Clusters of Differentiation (CD) molecules play essential roles in AML progression. This study aims to investigate the effect of COVID-19 on the expression of circulating miRNA and CD molecules in AML. This cross-sectional study recruited 32 AML patients and 20 controls. Blood samples were collected and analysed using molecular cytogenetic, miRNA/mRNA expression, and flow cytometry techniques. The expression of miRNAs varied significantly between patients with AML and control individuals. The co-expression of these miRNAs was higher (P < 0.05), indicating that the presence of one miRNA led to increased expression of other miRNAs. A differential correlation was observed between miRNAs and CD markers. Additionally, miRNA 16, miRNA 21, and miRNA 221 showed significant downregulation (P < 0.05 and P < 0.01, respectively) in AML patients with COVID-19 infection compared to those without a disease. Interestingly, this study identified a higher expression level (P < 0.01) of miRNA 137 as a novel biomarker for AML patients. Moreover, the expression of miRNA 137 showed a high correlation (P < 0.05) with most of the CD markers examined in this study and FISH features data. Furthermore, a strong correlation (P < 0.01) was observed between CD markers and miRNA among AML patients with positive and negative COVID-19 infection. These data demonstrated that COVID-19 contributed to increased expression of microRNAs in AML patients. MicroRNA 137 was identified as a novel microRNA that exhibited significant differences between patients and healthy individuals, highlighting its role in AML pathogenesis.
摘要：
急性髓性白血病（AML）是一种侵袭性白血病，其特征是原始细胞增殖不受控制。miRNA和分化簇(CD)分子在AML进展中发挥重要作用。本研究旨在探讨COVID-19对AML循环miRNA和CD分子表达的影响。这项横断面研究招募了32名AML患者和20名对照。收集血液样本并使用分子细胞遗传学进行分析，miRNA/mRNA表达，和流式细胞术技术。miRNA的表达在AML患者和对照个体之间显著不同。这些miRNA的共表达量较高（P<0.05），表明一个miRNA的存在导致其他miRNA的表达增加。在miRNA和CD标记之间观察到差异相关性。此外,miRNA16、miRNA21和miRNA221在患有COVID-19感染的AML患者中显示出显着下调（分别为P<0.05和P<0.01）。有趣的是,这项研究发现miRNA137的较高表达水平(P<0.01)是AML患者的新生物标志物。此外，miRNA137的表达与本研究中检查的大多数CD标记和FISH特征数据具有高度相关性(P<0.05)。此外，在COVID-19感染阳性和阴性的AML患者中，CD标志物与miRNA之间存在强相关性（P<0.01）。这些数据表明，COVID-19有助于AML患者微RNA的表达增加。MicroRNA137被鉴定为一种新的microRNA，在患者和健康个体之间表现出显著差异。强调其在AML发病机制中的作用。
