PDF(Pubmed)
Abstract：
Bacteria induced metamorphosis observed in nearly all marine invertebrates. However, the mechanism of bacteria regulating the larvae-juvenile metamorphosis remains unknown. Here, we test the hypothesis that c-di-GMP, a ubiquitous bacterial second-messenger molecule, directly triggers the mollusc Mytilus coruscus larval metamorphosis via the stimulator of interferon genes (STING) receptor. We determined that the deletion of c-di-GMP synthesis genes resulted in reduced c-di-GMP levels and biofilm-inducing activity on larval metamorphosis, accompanied by alterations in extracellular polymeric substances. Additionally, c-di-GMP extracted from tested varying marine bacteria all exhibited inducing activity on larval metamorphosis. Simultaneously, through pharmacological and molecular experiments, we demonstrated that M. coruscus STING (McSTING) participates in larval metamorphosis by binding with c-di-GMP. Our findings reveal that new role of bacterial c-di-GMP that triggers mussel larval metamorphosis transition, and extend knowledge in the interaction of bacteria and host development in marine ecosystems.
摘要：
在几乎所有海洋无脊椎动物中都观察到细菌诱导的变态。然而,细菌调节幼虫-幼体变态的机制尚不清楚。这里,我们检验了c-di-GMP的假设，一种普遍存在的细菌第二信使分子，通过干扰素基因（STING）受体的刺激器直接触发软体动物Mytiluscoruscus幼虫变态。我们确定c-di-GMP合成基因的缺失导致c-di-GMP水平降低和生物膜对幼虫变态的诱导活性。伴随着细胞外聚合物的改变。此外,从测试的不同海洋细菌中提取的c-di-GMP均表现出对幼虫变态的诱导活性。同时,通过药理学和分子实验，我们证明了M.cuscusSTING（McSTING）通过与c-di-GMP结合参与幼虫变态。我们的发现揭示了细菌c-di-GMP引发贻贝幼虫变态转变的新作用，并扩展了海洋生态系统中细菌与宿主发育相互作用的知识。
