Abstract：
The widespread use of surfactants raise challenges to biological wastewater treatment. Anaerobic ammonium oxidation (anammox) process has the potential to treat wastewater containing anionic surfactants, but the response of anammox consortia at the molecular level under long-term exposure is unclear. Using high-throughput sequencing and gene quantification, combined with molecular docking, the effect of sodium dodecyl sulfonate (SDS) on anammox consortia were investigated. Levels of reactive oxygen species (ROS) might be lower than the threshold of oxidative damage, while the increase of lactate dehydrogenase (LDH) represented the cell membrane damage. Decreased abundance of functional genes (hdh, hzsA and nirS) indicated the decrease of the anammox bacterial abundance. Trace amounts of N-acyl homoserine lactone (AHL, C6-HSL, C8-HSL and C12-HSL) contained in influent could induce endogenous quorum sensing (QS), which could regulate the correlation between functional bacteria to optimize the microbial community and strengthen the resistance of anammox consortia to SDS. In addition, the proliferation of disinfectant resistance genes might increase the environmental pathogenicity of sewage discharge. This work highlights the potential response mechanism of anammox consortium to surfactants and provides a universal microbial-friendly bioenhancement strategy based on QS.
摘要：
表面活性剂的广泛使用对废水生物处理提出了挑战。厌氧氨氧化（anammox）工艺具有处理含阴离子表面活性剂废水的潜力，但是在长期暴露下，厌氧氨氧化在分子水平上的反应尚不清楚。利用高通量测序和基因定量，结合分子对接，研究了十二烷基磺酸钠（SDS）对厌氧氨氧化体系的影响。活性氧（ROS）的水平可能低于氧化损伤的阈值，而乳酸脱氢酶（LDH）的增加代表了细胞膜的损伤。功能基因丰度降低(hdh，hzsA和nirS）表明anammox细菌丰度降低。微量N-酰基高丝氨酸内酯(AHL，C6-HSL,进水中所含的C8-HSL和C12-HSL)可以诱导内源性群体感应(QS)，可以调节功能菌之间的相关性，优化微生物群落，增强厌氧氨氧化菌对SDS的抗性。此外,消毒剂抗性基因的增殖可能会增加污水排放的环境致病性。这项工作强调了厌氧氨氧化菌对表面活性剂的潜在响应机制，并提供了基于QS的通用微生物友好型生物增强策略。
